35° DANIEL I, ARNON 



TABLE I 



Effect of Vitamin K3 and Phenazine Methosulphate (PMS) 



ON Photophosphorylation by Aged Cell-Free Preparations of Chromatium 



[Chromatophores (P) and Supernatant Fluid (S)] 



(Ogata, Nozaki, and Arnon [91]) 



* P and S were stored separately in Treatments i and 2 and together in Treat- 

 n\ents 3 and 4. 



Each vessel contained, in a final volume of 3-0 ml., cell-free preparation 

 containing 0-3 mg. bacteriochlorophyll, and the following in micromoles : tris 

 buflFer, pH 7-8, 80; MgCL, 5; K,H^^PO,, 10; and ADP, 10. o-i ^mole each of 

 vitamin K3 (2-methyl-4-amino-i-naphthol hydrochloride) and PMS were added 

 as indicated. Gas phase was argon. The reaction was carried out at 20° for 30 min. 

 and stopped bv adding 0-3 ml. of 20^',, TCA to each vessel. Illumination 35 000 

 Lux. 



TABLE II 



Effect of Inhibitors on Photophosphorylation by Chromatium Particles 



(Ogata, Nozaki, and Arnon [91]) 



rp ^ ^ umoles P esterified/ 



ireatment '^ , , . ,,/u 



mg. chlorophyll /hr. 



Control 75 



10^ M dinitrophenol 52 



5 X 10 ^ M o-phenanthroline 50 



Control 126 



Antimycin A, 10 /ug. 119 



Gramicidin, 40 ju,g. 119 



10^ M methylene blue 100 



10 ^ M /)-chloromercuribenzoate 14 



Each vessel contained, in a final volume of 3-0 ml., cell-free preparation (PS) 

 containing o • 2 mg. bacteriochlorophyll and the following, in /xmoles : tris buffer, 

 pH 7-8, 80; MgClo, 5; KoH^-POj," 15; ADP, 15; vitamin K3 (2-methyl-i,4- 

 naphthoquinone), 0-3; and phenazine methosulphate, o-i. Inhibitors were added 

 as indicated. Other conditions were the same as described for Table I. 



