SALMONELLA ANTIGENS 



111 



TABLE 8-5 



Specific Inhibition of Precipitation of Salmonella Antigens by 

 Anti-5. typhosa Antiserum* 



* Staub et al., 1959. Numbers indicate per cent inhibition. 



^ Polysaccharide extracted from S. typhosa. 



" PsTy oxidized with periodic acid. 



<^ Polysaccharide from 5. schottmuelleri (formerl}- paratyphoid B) = S. para- 

 typhi B. 



« Somatic antigens 9 and 12 of the KaulTman — White scheme. The italic number 

 indicates the antigen which characterizes group D, the group that includes 

 5. typhosa. 



S. typhosa after treatment with periodic acid. The reason for inckid- 

 ing such oxidized antigens in the studies is that periodic acid destroys 

 substances possessing two adjacent hydroxyl groups, such as terminal 

 ghicose or galactose. Terminal 3,6-dideoxyhexoses, however, do not 

 possess such a combination of hydroxyls and are not attacked. 



From the results obtained with the horse anti-typhoid serum shown 

 in Table 8-5, Staub et al. (1959) concluded that tyvelose is the 

 terminal sugar of antigen 9 and rhamnose that of antigen 12. 



It will be seen from Table 8-5 that the results obtained with the 

 rabbit serum were quite different from those of the horse serum. 

 The precipitation of the polysaccharide of S. typhosa (PsTy) by 

 horse anti-typhoid was inhibited significantly only by rhamnose and 

 tyvelose, whereas these sugars inhibited precipitation of the same 

 antigen by rabbit anti-typhoid very poorly. Glucose was much more 

 active with rabbit serum. The difference in inhibition of precipitation 

 of the polyoside of 6^. scJwttiniiellcri (PsPtB) was even greater. It 

 was therefore concluded that antigen 12, common to S. typhosa and 



