THE INTERPHASE NUCLEUS 



Stresses, must swiftly have undergone a process corresponding to the 

 comparatively slow evolution of natural prophase. The obvious method 

 of finding whether this is so, is to examine samples of the material at 

 intervals while it is being prepared. Such observations have been made 

 subsequently by Lamb^^^ ^^^ who has repeated the various methods of 

 disintegrating isolated nuclei. He has concluded that the first effect of 

 them all is to draw out the whole nucleus into elongated bodies, which 

 later break up into the smaller threads. These cannot thus be regarded 

 as morphologically equivalent to chromosomes. Nor was Lamb able 

 to confirm the double nature of these threads by electron micrography. 

 Thus the conclusion seems unavoidable that isolated chromosomes 

 have not yet been artificially prepared from resting nuclei. 



REFERENCES 



Nucleus of chick and mammalian cells in living tissue cultures 



1 Vanderlyn, L., Science, 104 (1946), 514 



^ Chambers, R., and Fell, H. B., Proc. Roy. Soc. Land., b, 109 (1931), 380 



Chromosomes and the deoxynucleoproteins 



^ White, M. J. D., Animal Cytology and Evolution, Cambridge, 1945 



•• Gray, J., Brit. J. exp. Biol., 5 (1927), 102 



* Lewis, W. H., and Lewis, M. R., General Cytology, Ed by C. V. Covvdrey, 

 Chicago, 1924 



•^ Strangeways, T. S. p., and Canti, R. G., Quart. J. micro. Sci., 71 (1927), i 



" Flemming, W., Z^llsubstanz, Kern, and ^elltheilung, Leipzig, 1882 



" Lewis, M. R., Johns Hopk. Hosp. Bull., 34 (1923), 373 



^ Fell, H. B., and Hughes, A. F., Quart. J. micr. Soc, 90 (1949), 355 

 1" Tellyesniczky, K., Arch. Mikr. Anat., 66 (1905), 367 

 11 Shinke, N., Cytologia, Fujii Jub., i (1939), 449 

 '^ WiGGANS, R. G., Amer. J. Bot., 8 (1921), 30 

 '^ Weber, F., Protoplasma, 2 (1927), 305 

 '* Serra, J. A., Symp. quant. Biol., 12 (1947), 192 

 1* Shiwago, p., Biol. Zbl; 46 (1926), 679 

 1* Richards, A., BioL Bull., 63 (1917), 1 13 

 '" Kater, J. McA., Z- ZMforsch, 5 (1927), 263 

 i«—,t6iW, 6(1928), 587 

 ^^ Lewis, W. H., Anat. Rec, 97 (1947), 433 

 ^^ Ris, H., and Mirsky, A. E., J. gen. Physiol., 33 (1949), 125 

 -1 Arnesen, K., Goldsmith, Y., Dulaney, A. D., Cancer Res., 9 (1949), 609 

 ^2 LuDFORD, R. J., Smiles, J,, and Welch, F. V.,J. roy. micr. Soc, 68 (1948), i 

 ^* — , and Smiles, J., ibid, 70 (1950), 186 

 '-* Ris, H., and Mirsky, A. E., J. gen. Physiol., 33 (1949), 489 

 ^* Brown, G. L., Callan, H. G., and Leaf, G., Nature, Lond., 165 (1950), 600 

 2* Vendrely, R., and Vendrely, C, Experentia, 4 (1948), 434 

 " Davidson, J. N., and McIndoe, W. M., Biochem. J., 45 (1949), Proc. XVI 

 28 Bounce, A. L., Tishkoff, G. H., Barnett, S. R., and Freer, R. M., J. gen. 



Physiol, 33 (1950), 629 

 -' BorviN, A., Vendrely, R., and -Vendrely, C, C. R. Acad. Sci., Paris, 226 



(1948), 1061 

 '» Mirsky, A. E., and Ris, H., Nature, Lond., 163 (1949), 666 

 ^•^ — , — , J. gen. Physiol., 34 (1951), 451 

 3' Zittle, C. a., and O'Dell, R. A., J. biol. Chem., 140 (1941), 899 



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