THE FUNDAMENTAL ORGANIZATION 



71 



from eight to fourteen hours, a positive reaction is obtained in all 

 forms in which the secondary nuclei are present (Fig. 3(3). Here 

 the nuclei and the embedding matrix of chromidia are intensely 

 stained. ( 'hromidia at other stages give varying shades of purple 

 depending apparently upon the condition of the organism. Nucleic 

 acid which is formed in the chromidia becomes concentrated in 

 the secondary nuclei; these obviously would resist the pepsin 

 digestion while the residue is dissolved. 



Fig. 36. 



-Arcella vulgaris. Growth of nucleic acid bodies in the chromidia! net. 

 (Original, X 500 and X 1000.) 



The problem of extranuclear chromatin, or chromidia, assumed 

 a novel theoretical significance with the development of Hartmann's 

 so-called polyenergid theory. Hartmann (1909) suggested a mor- 

 phological interpretation of Sachs "energid" or nucleus with its 

 sphere of influence, by suggesting an energid as a nucleus consisting 

 of two components, one the chromatin or idiogenerative component, 

 the other a centrosome or homologous structure (kinetic or loco- 

 motor component). In 1911 he distinguished three main types of 

 nuclei of Protozoa, viz., monoenergid, meroenergid and polyenergid 

 types. Monoenergid types are in Protozoa having one kind of cell 

 division as in most flagellated Protozoa. Meroenergid types are 

 forms, originally with two nuclei, one of which has lost the idio- 



