AEROBIC PHOSPHORYLATION IN R. RUBRUM 



165 



4 6 8 10 



Fraction 



Fig. 1. Activity profile of Crude Dark Extract Centri- 

 fuged Forty Minutes at 35,000 rpm-Crude dark ex- 

 tract, 5.9 mg protein, was centrifuged in a sucrose 

 density gradient; fractions were collected and analyzed 

 as described in the text. The first fraction represented 

 the bottom fraction of the tube. 



In order to translate arbitrary units into activity 

 stated in terms of each undiluted fraction: one OD28O 

 unit (open triangles) represents an optical density of 

 0.65 at 280 m/i, one succinic dehydrogenase unit 

 (squares) represents 2.2 m/imoles indophenol reduced/ 

 ml/min, one DPNH oxidase unit (triangles) represents 

 6.6 m/imoles DPNH oxidized/ml/min, and one M-6-P 

 unit (circles) represents 2.1 m/ymoles M-6-P formed/ 

 ml/min. 



