166 



METABOLISM AND PHYSIOLOGY 



Fig. 2. Activity profile of Crude Dark Extract Centri- 

 fuged Sixty Minutes at 35,000 rpm— Crude extract, 8.2 

 mg protein, was treated in the manner outlined in Fig. 

 1 (except that the centrifugation time was extended to 

 sixty minutes). The actual values of the arbitrary 

 units were (in terms of undiluted fractions): forOD280 

 (open triangles), one unit represents an optical density 

 of 0.865 at 280 m//, for succinic dehydrogenase 

 (squares) 2.2 m^umoles indophenol reduced/ml/min, 

 for DPNH oxidase (triangles) 3.7 m^t/moles DPNH ox- 

 idized/ml/min, and for M-6-P (circles) 1.0 m//mole 

 M-6-P formed/ml/min. 



