174 METABOLISM AND PHYSIOLOGY 



phorylation system purified by sucrose density gradient centrifugation 

 are described. 



ACKNOWLEDGMENTS 



I am indebted to Dr. George Drysdale for his valuable advice and loan of 

 chart paper for the recording equipment. 



REFERENCES 



1. Geller, D. M., Oxidative phosphorylation in extracts of Rhodospirillum 

 rubrum. J. Biol. Che?)}., 237, 2947 (1962). 



2. Britten, R. J., and Roberts, R. B., High-resolution density gradient sedi- 

 mentation analysis. Science, 131, 32 (1960). 



3. Martin, R. G., and Ames, B. N., A method for determining the sedimenta- 

 tion behavior of Enzymes: Application to protein mixtures. J. Biol. Chem., 

 236, 1372 (1961). 



4. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., Protein 

 measurement with the folin phenol reagent. J. Biol. CIicdi., 193, 265 (1951). 



5. Block, R. J.,Durrum,E. L.,and Zweig, G.,A Maiiuol of Paper Chromatog- 

 raphy and Paper Electroplioresis . Academic Press, Inc., New York (1955), 

 p. 133. 



6. Lardy, H. A., Johnson, D. , and Mc Murray, W. C, Antibiotics as tools for 

 metabolic studies. I. A survey of toxic antibiotics in respiratory, phosphory- 

 lative and glycolytic systems. ^ re/?. Biochem. Biophys., 7S, 587 (1958). 



7. McMurray, W. C, and Begg, R. W., Effect of valinomycin on oxidative phos- 

 phorylation. A re/?. Biochem. Biophys., 84, 546 (1959). 



8. Heytler, P. G., and Prichard, W. W., A new class of uncoupling agents— 

 carbonyl cyanide phenylhydrazones. Biochon. Biophys. Res. Comnnai., 7, 

 272 (1962). 



9. Baltscheffsky, M. , On light-induced phosphorylation and oxidation of reduced 

 diphosphopyridine nucleotide in /?/?oc/os/)/r?7Z//;;? rubrum. Acta Chem.Scand., 

 15, 215 (1961). 



10. Tuttle, A. L., and Gest, H., Subcellular particulate systems and the photo- 

 chemical apparatus of /itorfos/^rnZZw;;^? rubrum. Proc. Natl. Acad. Sci. U. S., 

 45, 1261 (1959). 



11. Horio, T., and Kamen, M. D., Observations on the respiratory system of 

 Rhodospirillum rubrum. Biochemistry, 1, 1141 (1962). 



