290 



ELECTRON TRANSPORT 



0.4 



E 



O 

 m 



lO 



0.3- 



0.2 



0.1 



0.0 



NON ADDITION .-hATABRINE 



+ ANTIMYCIN A 



-l-ADP, R +ADP,ANTIMYCIN A 

 4-ADP.R,ANTIMYCIN A.ATABRINE 



12 3 4 



TIME (MINUTES) 



Fig. 10. Effect of various substiinces on photooxidation of cyto- 

 chrome Cp by R. rnbniiii chromatophores. Reaction naixture 

 (1.20 ml.) "contained components as follows: 0.033 M Tris (j^H 

 8.0), 3.3% sucrose, 0.008 M MgCl2, 0.008 M ADP, 0.008 M Pi, 

 4.1 X 10-4 M KCN, 8 x 10"^ m atabrine, 1 y antimycin A, approx. 

 2 X 10-5 M cytochrome C9 and R. nibrum chromatophores 

 (A 880 mil " 0.42). Reactions were carried out at 20° C in light of 

 approximately 1,000 ft-candles. Difference absorbancy was made 

 between the two cuvettes containing the reaction components 

 with and without cytochrome co. 



