420 



PHOTOMECHANICAL CONSIDERATIONS 



estimated absorption of the sample would rise more steeply than shown 

 in Fig. 6 as the wavelength decreases from 850 to 750 m/n. Even with 

 the correction, it is clear that the efficiency of light at 812, 825, 837, 

 and 850 m/i is higher than the efficiency of light at 774, 786, and 798 m/u. 



- 20 



16 



700 750 800 



X IN m^ 



850 



12 ^o 



o 



< 

 I 



CVJ 



< 



-4 ex 



Fig. 6. Absorption (1 - T) spectrum (solid curve) and action spectrum of cyto- 

 chrome oxidation (closed circles) for a suspension of Cl^s. clhylicmii. The ab- 

 sorption spectrum has been corrected for light scattering as described in the 

 text. The points of the action spectrum are ratios of initial rate of absorbancy 

 change at 422 rafi to quantum intensity of actinic light. 



