CYTOCHROME SPECTRA 



481 



1.0 

 0.9 

 0.8 

 0.7 



S 0.6 



i 0.5 



o 



(/) 



CD 



< 0.4 



0.3 

 0.2 



0.1 



4 



3 



0.2 



I 



-0.1 



400 



450 



500 



550 



600 



WAVELENGTH (m;j) 



Fig. 2. Rps. palustris cytochrome C2 ferro- and ferri- absolute 

 spectra (A) and ferro-minus-ferri- difference spectrum (B) at pH 7 

 (50 mM potassium phosphate buffer), d = 1.0 cm. The heme concen- 

 tration for the visible region spectra is 21.6 ^M and for the Soret 

 region is 7.3 /iM. 



