484 



APPENDIX 



400 450 500 550 



WAVELENGTH imp) 



600 



650 



Fig. 4. R. rubrum cytochromoid C (RHP) ferro- and ferri- absolute spectra 

 (A), ferro-minus-ferri- difference spectrum (B), CO-ferro- absolute spectra 

 (C), and CO-ferro-minus-ferri- difference spectrum (D) at pH 7 (50 mM potas- 

 sium phosphate buffer), d = 1.0 cm. For the CO spectra the gas phase is 1 

 atmos. CO. The heme concentration for the visible region spectra is 29.1 |UM 

 (14.55 fiM cytochrome concentration) and for the Soret region is 9.7/^M for A 

 and 3.23 ilM for B, C and D. 



