486 



APPENDIX 



400 450 500 550 



WAVELENGTH (m;;) 



600 



650 



Fig. 5. Rps. Ijolus tri s cytochromoid C (RHP) ferro- and ferri- absolute spectra 

 (A), ferro-minus-ferri- difference spectrum (B), CO-ferro- absolute spectra 

 (C) and CO-ferro-minus -ferro- difference spectrum (D) at pH 7 (50 mM potas- 

 sium phosphate buffer), d = 1.0 cm. For the CO spectra the gas phase is 1 

 atmos. CO. The heme concentration for the visible region spectra is 30 ^M 

 (15 fiM cytochrome concentration) and for the Soret region is 10 /iM for A and 

 3.33 ^Mfor B, C and D. 



