488 



APPENDIX 



400 



450 500 550 



WAVELENGTH (m;j) 



600 



650 



Fig. 6, Chromatium stTcLin D cytochromoid C (RHP) ferro- andferri- abso- 

 lute spectra (A), ferro-minus-ferri- difference spectrum (B), CO-ferro- ab- 

 solute spectra (C) and CO-ferro-minus-ferro- difference spectrum (D) at 

 pH 7 (50 mM potassium phosphate buffer), d = 1.0 cm. For the CO spectra 

 the gas phase is 1 atmos. CO. The heme concentration for the visible region 

 spectra is 29.7 /iM ( 14.85 jUM cytochrome concentration ) and for the Soret 

 region is 9,9 fuM for A and 3.3 jLlM for B, C and D. 



