MEDIA FOR ANAEROBIC GROWTH 505 



Rhodopseudomonas palustris. 



Synthetic medium. After Keane, Zahalsky, Hutner and Lubart, p, 

 163 in Studies on Micvoalgae and Photosynthetic Bacteria (Japanese 

 Soc. of Plant Physiologists, ed.), Univ. Tokyo Press, 1963. 

 MgS04 . 7 H2O 

 CaCl2 

 (NH4)2S04 

 Na2-glycerophosphate • 5 H2O 



(mixture of a and y3 isomers) 

 Sodium acetate • 3 H2O 

 Tripotassium citrate • H2O 

 L- glutamic acid 



DL-malic acid, neutralized with KOH separately 

 L-histidine (free base) 

 L- tyrosine 

 Monobutyrin 

 /?-aminobenzoic acid 

 Homocysteine thiolactone • HCl 

 "Metals": 



Fe 



Mn 



Zn 



Mo 



Cu 



Co 



B 



V 



Se 0,036 mg 



Glass-distilled water to 1000 ml, 

 pH: 6.2 to 6.5 

 Incubation temperature: 30-34°C, 



Rhodops endo uiojias gelatinosa. 



After Siegel and Kamen, J. Bacterial., 59, 693 (1950). 



Stock Cultures: deep stabs in a medium containing 0.3% yeast 

 extract (Difco), 0.3% peptone and 1% agar in tap water. 



Basal Medium 



(NH4)2S04 1.0 g 



MgCl2 0.2 g 



CaCl2 • 2 H2O 0.04 g 



KH2PO4 2,0 g 



H3PO4, 6 N 3.6 ml 



Thiamine • HCl 4.0 mg 



