506 APPENDIX 



Nicotinic acid 4.0 mg 



Biotin 5.0 /i g 



Bicarbonate solution 40.0 ml 



(5 g NaHCOs + 5 g KHCO3 per 100 ml). Added 

 aseptically after separate sterilization. 

 Tap water to 1000 ml. 

 The basal medium is supplemented with desired substrate (isopro- 



panol (0.1-0.2%), acetate, acetoacetate, pyruvate, glycerate). The final 



medium should have a pH of 7 to 7,5. 

 Incubation temperature: 30°C. 



Rhodonite rob ium vannielii. 



After Duchow and Douglas, J. BacterioL, 58, 409 (1949); modified 

 by Morita and Conti, Arch Biochem. Biophys., 100, 302 (1963), 

 NaHC03 

 NaCl 



(NH4)2S04 

 K2HPO4 

 MgS04 • 7 H2O 

 Na2S • 9 H2O 

 Organic substrate 



(Ethanol, propanol, butanol, acetate, propionate, 

 butyrate, valerate, caproate or lactate) 

 Yeast extract 

 Water 

 pH adjusted to 7.0 with H3PO4. 

 Incubation temperature: 28-30°C. 



PURPLE SULFUR BACTERIA 



The purple sulfur bacteria most commonly used in biochemical 

 investigations belong to the genus Chromatium. These organisms 

 can be readily grown either with carbon dioxide as the sole carbon 

 source or with organic compounds. To obtain satisfactory growth in a 

 reasonable time period, the media currently in use are sown with 

 relatively large inocula (10-20%), 



Stock cultures: stabs in medium B specified below; sufficient agar 

 is added to solution 1 before autoclaving to give a final concentration 

 in the complete medium of 1,5% (w/v). 



Chromatin)//. 



A. Inorganic medium used by R, Bartsch (unpublished). See also: New- 

 ton and Kamen, Biochi/n. Biophys. Acta, 21,11 (1956), and Newton, p. 



