MEDIA FOR ANAEROBIC GROWTH 507 



73 in Methods in Enzymology (S. P, Colowick and N. O. Kaplan, eds.,), 

 Vol. V, Academic Press, New York, 1962. 



NaCl 10.0 g 



K2HPO4 0.5 g 



KH2PO4 0.5 g 



NH4CI 1.0 g 



MgCl2 • 6 H2O 0.5 g 



CaCl2 0.05 g 



NaHC03 2.0 g 



FeCl3 • 6 H2O 0.005 g 



Na2S203 • 5 H2O 2.0 g 



Na2S . 9 H2O 1.0 g 



Tap water to 1 liter; final pH adjusted to 7.8-8.0. If distilled or 

 demineralized water is used, 1 ml of the trace elements solution de- 

 scribed by Larsen {J. Bacteriol., 64, 187 (1962)) is added per liter of 

 medium. 



When molecular hydrogen is used as the accessory electron donor, 

 thiosulfate and sulfide are omitted from the medium. 

 Incubation temperature: 30-35 °C, 



B. Inorganic medium used by R. C. Fuller, based on the medium of 

 Hendley (J. Bacteriol., 70, 625 (1955)). 



Solution 1 

 NaCl 

 NH4CI 

 KH2PO4 

 MgCl2 

 CaCl2 



Trace elements solution (see below) 

 Cone. HCl 

 Distilled water to 500 ml. 



Solution 2 



Na2C03 12.76 g 



Na2S203 6.0 g 



Ethylenediamine tetraacetic acid 0.2 g 



Na2S . 9 H2O 0.1 g 

 Distilled water to 500 ml. 



Autoclave solutions 1 and 2 separately and cool. Add 1.0 ml of 



cone. HCl to 1 and pour solution 2 into 1 very slowly. Final pH should 



be between 7,8 and 8.0. 



