508 



440.0 mg 



240.0 mg 



13.5 mg 



16.5 mg 



880.0 mg 



Versenol iron solution (see below) 110.0 ml 



Distilled water to 1 liter. 



Versenol Iron Solution 

 59 g of ethylenediamine tetraacetic acid (Versenol) are dis- 

 solved in 500 ml distilled water. 24.9 g of FeS04 • 7 H20 are 

 added, the solution diluted to 1 liter with distilled water, and 

 then aerated overnight. The final pH should be approximately 9.7, 



C. "Heterotrophic" synthetic medium used by R. C. Fuller. 



Stock Salt Solution 

 CaCl2 0.25 g 



NH4CI 1.2 g 



MgS04 * '^ H2O 0,66 g 



NaCl 20,0 g 



Hutner's trace elements solution 1,0 ml 



(see below) 

 Distilled water to 1000 ml, 



Hutner's Trace Elements Solution 

 (J. BacterioL, 52, 213 (1946)) 

 Ethylenediamine tetraacetic acid 50,0 g 



ZnS04 • 7 H2O 22.0 g 



H3BO3 11.4 g 



MnCl2 • 4 H2O 5.1 g 



FeS04 • 7 H2O 5.0 g 



C0CI2 • 6 H2O 1.6 g 



CUSO4 • 5 H2O 1.6 g 



(NH4)6Mo7024 • 4 H2O 1.1 g 



Boil in 750 ml distilled water, cool slightly and bring to pH 

 6.5-6.8 with KOH (do notuseNaOH). The clear solution is diluted 

 to 1000 ml with distilled water and should have a green color 

 which changes to purple on standing. Stable for at least one year. 



Final Medium 

 Solution I 120 ml of stock salt solu- 



tion diluted to 800 ml with 

 distilled water. 



