STAINING TECHNIQUES 5-12 



Results: Chromosomes - purple -red 

 Centrosome - 

 Nucleolus - pale blue 

 Cytoplasm - pale blue 

 8. Sudan IV. Use a saturated solution of Sudan IV in 70% alco- 

 hol which has been standing 24 hours or overnight before 

 using. 



a. Fixation; Navashin 5 minutes or formalin vapor 10 min- 

 utes. 



b. Tap H2O wash, 10-15 minutes. 



c. 50% alcohol, 1-2 minutes. 



d. Stain in Sudan IV in 70% 45 minutes. 



e. 70% alcohol, rinse. 



f . Distilled water wash, 2 minutes. 



g. Aqueous solution of fast green, 2-3 minutes, 

 h. Distilled H2O, rinse. 



i. Mount in Karo syrup (blue label) and ring with gold 



sizing. 

 Results: Chromosomes, sometimes nucleolus, as well as 

 cytoplasmic bodies take on the stain. 



