CYTOLOGy 



6-6 



and found that it contained two bodies which he called the "chromo- 

 somes." He stated that each cell contained two "chromosomes'* 

 which divided by longitudinal splitting. He stated that the haploid 

 "chromosomes" fuse end to end to form the diplophase, rather than 

 associating to form a pair of chromosomes according to the usual 

 method. The life cycle described by Badian is shown in fig. 6-2, 

 copied from his paoer. 



dD © 



© 

 



Fig. 6-2 Yeast Life Cycle as Described by Badian. This Fig- 

 ure is Copied from his Paper Showing Spore Formation from the Dip- 

 loid Cell (a-g), and the Fusion of Two Haploid Cells to Reconsti- 

 tute the diploid (h, i, j). However, it is not Clear Whether these 

 Bodies are the Chromosomes, as Badian Thought, or the Heterochro- 

 matin Carried»in the Centrosome. 



The centrosome contains two rod-shaped bodies which stain 

 well with Feulgen and aceto-orcein (fig. 5-3). They may be the bod- 

 ies described by Badian as "chromosomes" which he states fuse 

 end to end to produce the diplophase. However, the vacuoles also 

 fuse following copulation. Harper showed that fusion of the nuclei 

 in Phyllactinia is initiated by fusion of the centrioles, followed by 

 fusion of the nuclear vacuoles and sjmapsis of the chromosomes. 



