6-35 



THE YEAST CELL 



first hour in the nutrient broth, the cells enlarge and show a faintly 

 staining pink volutin deposit covering the chroniosomal threads. As 

 the cells continue to swell preparatory to division, the nuclear vacu- 

 ole becomes much enlarged and the chromosomes begin to take the 

 stain more deeply. In this stage, the shape and form of the chromo- 



4 *^j«# 



^^- MM- 



"*>« 



Fig. 6-25 Volutin coated chromosomes are visible within the vacuole. 



somes can be clearly distinguished, and they can be made out as 

 single strands or pairs of small, rather compact rods. As the vacu- 

 ole enlarges still more, two nucleoli also appear, but during this 

 period, they do not stain. About the time the first buds come up, 

 one or two nucleoli are visible as large, light green, refractile bodies 

 in the nuclear vacuole. The chromosomes now appear as a large 

 number of individual bodies on the inner periphery of the vacuole. 



I 



