ADAPTATION TO PANTOTHENATE DEFICIENCY 



22-22 



Table 22-3 



The Number of Cells in Duplicate Cultures 

 of ff215^ in Pantothenate- free Medium 



GROWTH OF THE RECESSIVE CULTURE IN THE 

 ABSENCE OF PANTOTHENATE 



In general, when culture #2154 is inoculated into pantothenate- 

 free medium, there is slow growth for a month or longer and then 

 the culture "adapts" and grows fairly rapidly. When transfers are 

 made from the culture after completion of growth into new tubes 

 of test medium, a rapid rate of growth is maintained. 



To study the nature of the adaptation in pantothenate -free me- 

 dium, the following exoeriment was set up: Several tubes of panto- 



