INTERCHROMOSOMAL TRANSFER 25-2 



2 : 2 segregations occurred at the G/g, IN/in, and PN/pn loci. In 

 addition to transformations at the AD/ad locus, three of the cultures 

 were S and one s and all four cultures were thiamin and pyridoxine 



5756 o^ ^ PINK 9 in pn pj S ih 



5735 9 AD WHfTE & \H PN FY /i TH 



5831 ^ AO WHITE G W pn FY S TH 



58X « aJ PINK a IN Pf^ Pr S ih 



in PN PY J ih 



jojc ex oa rinn o 



5Q35 a^ AO WHITE & 



5831- a aJ PWir 9 in pn^ pj xJ TH 



5063 ot y^O WHITE G JN PN FT /^ TH 



5864- « AD WHITE & IN PN PY 5 TH 



5665 ^ ^ PINK g in pn py A M 



5866 H W PINK 9 /n pn g. 5 U 



567i ji a^ PINK g in on Pf yi TH 



5872 « AD WHITE G JN PN py 5 TH 



58^ cK AD WHITE G it, PN PY J M 



567f ti ad PINK 9 IN pn p/ /i M 



5907 «J PINK G in pn py 5 TH 



J?06 arf PINK 9 IN PN FT /i TH 



5909 AD WHITE G in pn py xj <a 



3910 AD WHITE 9 IN PN PT 5 tk 



5919 a AD WHITE G IN PN PT ^ TH 



5920 o< ^ PINK Q in pn py 5 t/, 



5921 « ad PINK 9 IN pn Pr /J TH 



5922 at d PINK G in PN fy 5 ik 



5979 n AD WHITE G IN PN Pf ^ TH 



59d0 * ad RNK 9 w pn py ^ TH 



5981 o< a<i PINK 9 in pn pV 5 th 



5982 oc */ PINK G IN PN PY 5 M 



60f0 ? AD WHITI Q in pn PY ^ TH 



6041 ? AD WHITE G IN PN PY S TH 



60« ? AD WHITE G JN PN PY S TH 



6043 ? AD WHITE 9 in pn PY S TH 



Fig. 25-1 Tetrad analysis ot seven sample asci from a hybrid 

 heterozygous for six alleles on one chromosome. 



independent. Mating types were also disturbed. The test for mating 

 type is ordinarily made by testing all 4 cultures with a single vigor- 

 ously copulating haplophase culture and those which copulate are 

 designated as opposite sex to the tester, while those which do not, 

 are marked the same sex as the tester. If the tests are not clear- 

 cut, a second test using the opposite mating type usually clears up 

 any ambiguity. However, No. 6040-43 was tested by mating to both 

 a and a mating types and only negative reactions were obtained in 

 every case. These cultures, therefore, were practically sterile. An 



