2 LOCAL ILSSl'E RKAClIVrrY 



Protocol i: I'pixi and lower rij^lil and Iclt areas ol tlic skin oi llic 

 alxlonun ol Rabhiis i| i and i.|-2 were injecled each with 0.5 ex. ol 

 /.'. l\l>li()SHs ir\])ii( dincsi hioih cnhurc filtrate (strain I]). Twenty-lour 

 houis hiter Raijbil i.]-! showed no skin reactions. Rabbit \,\-2 sliglitly ])ink 

 reactions (2-)-) in the injected areas. At this time single intravenous injec- 

 tions ol ;} (.c. per kilo ol body weight ol the filtrate were given to both 

 labbiis. Ihe skin ol the animals was examined every hour alterwards. 

 Approximately two hoins alter the intravenous injections, blue discolora- 

 tion appeared at the site of previous skin injections in Rabbit 14-1. The 

 skin ol Rabbit 14-2 remained imchanged. The discoloration observed in 

 Rabbit i.pi rapidly increased until the reactions became extremely pro- 

 nounced in about loin- hours alter the intravenous injection. All four areas 

 were then dark blue in the center with a deep red zone at the periphery. 

 The skin over the hematomas was glossy and swollen. The size ol these areas 

 was considerable, the reaction in the upper right corner measuring 2x2 cm., 

 the lower right 3x2 cm., the upper left 4x2 cm., and in the lower left 

 corner 3 x 2.5 cm. The reaction described resembled a severe bruise. The 

 animal was killed 5 hours alter the intravenous injection and sections ol the 

 skin were made. Rabbit 14-2 showed no reaction in the course of twenty- 

 four hours folloAving the intravenous injection. 



Protocol 2 : Four areas of the skin of the abdomen of Rabbit 7-6 were 

 injected each with 0.25 c.c. of B. typhosus tryptic digest broth culture fil- 

 trate (strain T240) . Twenty hoins later there were moderate erythemas at 

 the site of skin injections. The rabbit was then injected intravenously with 

 the filtrate in a dose of 1 c.c. per kilo ol body weight. The previously in- 

 jected skin areas showed discoloration in about two hours after the intra- 

 venous injection. The reactions again, progressively, increased and about 

 four and one-half hours alter the intra\enous injection they were extremely 

 severe. The areas were hemorrhagic. They appeared dark blue, glossy and 

 swollen. The upper right corner reaction measured 3.5 x 3 cm., the lower 

 right 2 X 2.5 cm., the upper left 2x2 cm., and the lower left 1.5 x 3 cm. 

 Rabbit 7-6 ^vas re-examined twenty-four hours later. The sites were then 

 black with a dark red zone at the periphery. The size remained unchanged. 

 It appeared that the reaction reached its maximum size in about fi\e hours 

 after the intravenous injection. The healing of the hemorrhagic areas was 

 slow. Sloughs formed in about lorty-eight hours after the intravenous in- 

 jection were followed by scab formation, gradual separation ol scales and 

 scarring. The complete process ol healing took about eight days. 



Histological examinations of the hemorrhagic areas were made. 



Sections of these areas from Rabbit 14-1 obtained five hours alter the 

 intravenous injection can be described briefly, as follows: 



The general impression was that ol the severest type of hemorrhage and 

 necrosis. The skin was edematous in places. Some of the blood vessels were 

 ruptured. The subcutaneous tissue was engorged with l^lood. There was 

 also an extensive migration of polymorphonuclear neutrophile leucocytes. 

 There \vas obserxed pronounced necrobiosis of these cells located inside and 

 outside the blood vessels. Some of the blood vessels contained small parietal 

 thrombi. While it was clear that the process afTected the veins, it remained 

 questionable whether there was any primary injury to the arteries. Some of 



