INTRODUCTION 3 



the arteries Avere found normal. Others were ahiiost entirely occluded. The 

 obliteration, however, was probably secondary to the hemorrhagic infiltra- 

 tion outside the arteries. Occasionally, hyalinization was seen in the blood 



I'K.. 1. I'i\e sites of skin were injected each with 0.3 c.c. of T. D. l\ filtrate; 

 24 hours later rabbit recei\ed intra\enoiis injection of same filtrate. Dose was 2.5 

 c.c. per kilo of body weight. Appearance of reaction at site of prior skin injections 

 5 hours after intravenous injection. (Shwartzman, 19286.) 



\'essels. The hemorrhage and necrosis extended to the corium of the skin, 

 which -was thin and broken in places. 



The sections of the hemorrhagic areas of Rabbit 7-6 made twenty-four 

 hours after the intravenous injection were almost identical with the sections 

 of Rabbit 14-1. 



Early observations on routes of injection : 



It became obvious from the ttnidamental experiments that the 

 use of the intravenous route for the second injection ^vas an es- 

 sential recjuirement of the phenomenon (Shu'artzman, 1928/?, 

 iggofl) . Repeated skin Injections of the fikrate within tuenty- 

 four h()in~ interxal between injections did not resuk in the 

 hemorriiagic and necrotic type of reaction of the phenomenon. 



