22 LOCAL riSSUE REACTIVITY 



hours later by the iiitia\ eiioiis injection ol the reacting factors. 

 In some instances they obtained reactions in the non-prepared 

 ear. These reactions coirsisled ol petechial hemorrhages along 

 a large vein. In another group ol experiments they introduced 

 a fragment of sponge impregnated with 0.5 c.c. of B. coli ciilttire 

 lilt rate into a subcutaneous pocket of the abdominal ^vall of a 

 normal rabbit. The pocket was closed by an application of a 

 ligature. Twenty-foiu" hours later the sponge was removed and 

 macerated in saline. The fluid obtained Avas used for a prepara- 

 tory injection in the rabbit's ear. Animals thus prepared received 

 B. coli cidture filtrates. A few days later in the non-prepared and 

 prepared ears of positive raljbits, as Avell as in the non-prepared 

 and prepared ears of negative rabbits, there were noticed pur- 

 pmic lesions appearing along the capillary network of the margin 

 of the ear. This ptnpma ascended and finally involved the entire 

 ear. The ears became edematotis. The observations of Gratia 

 and Linz Avere corroborated l)y Pabst and Branham. 



In a few experiments of my own, the above observations with 

 B. coli and B. typhosus culture filtrates could not be reproduced 

 at Avill. The spreading seemed to occin- rather infrequently and 

 oirly in rabbits in \\hich the prepared ear reacted Avith unusual 

 severity. 



The spreading of the reactions to the non-prepared ear deserves, 

 however, special consideration inasmuch as it suggests the ten- 

 dency towards symmetrical distribution of hemorrhagic lesions 

 observed in the Schoenlein-Henoch syndrome of htmian hemor- 

 rhagic diathesis. 



It may be also in place to mention here Avidespread petechiae 

 extending from the local lesions observed sometimes with pneu- 

 mococcus cidture filtrates. 



In these experiments the skin sites were prepared either by 

 heterologous filtrates or pneimiococcus preparations (Shwartzman, 

 1932/) . Intravenously, the rabbits received large doses of autolyzed 

 pneumococcus glucose broth cid tines. In some rabbits there ap- 

 peared typical reactions which, however, frequently Avere very 

 large. If several sites Avere prepared, there Avas a decided tendency 

 for the reactions to become confluent. Petechial hemorrhages 

 Avere sometimes observed Avell beyond the prepared sites. It seems 

 to be of still more interest that in other instances it Avas possible 

 to obtain petechial lesions scattered over the greater portion of the 

 skin of the abdominal Avail, and of equal severity in prepared 

 and non-prepared sites. Thus far, the observations Avere made 



