40 LOCAL TLSSUE REACTIVITY 



ACIINI I'RINCII'I.KS Ol I',. Ml iriENSIS AM) B. ABORITS 



111 i<)2(), I attempted lo rcpiodiuc ihe j)henomenon of local 

 skill iea(tl\iiy to B. 'nicliloisi.s without success. In these experi- 

 ments there were emjiloyed tiyptic digest ascitic fluid broth cul- 

 tiues incubated for six days, aerobic and anaerobic double strength 

 broth cultures and "agar Avashings" filtrates. 



Kritschewski and Halperin (i9,'54) also failed to rejDroduce the 

 phenomenon to B. aborliis. They used three day old cultures in 

 plain l:)roth and injected 0.5 c.c. intradermally and 1 c.c. intra- 

 venously, of this material. In the same rabbits they were able, 

 however, to indtice the phenomenon with B. lyl)Ji()sii.s t^venty- 

 four hour old broth cidtures, thus proving that the failure was 

 due to lack of the active principles in B. abortus cultines and not 

 to resistance of the rabbits used to the phenomenon. 



/.uwerkalow, Fischbein and JuranofT (1935) carried out a series 

 of investigations on the active principles of B. abort us. Filtrates 

 of six to twenty-one day old cultmes gave negative residts. Con- 

 sidering my residts with meningococcirs ^vhereby it was shown 

 that there may be encoiuitered diHiculties in obtaining the active 

 j)rinciples in ffuid medium, they inidertook experiments with 

 "agar washings" filtrates. Thirty-six to forty-eight hour old cid- 

 tures of B. abortus of strain Schidgino on liver agar of pH 7.3 

 were suspended in saline (in proportion of i to 2 c.c. of saline to 

 a culture) . The strain Avas recently isolated and failed to gro^v in 

 the absence of a paraffin seal. Rabbits were prepared by four 

 simultaneous intradermal injections of 0.25 c.c. and reinjected 

 intravenously with 8-10 c.c. of the same material t^venty-four 

 hours later. Four hours after the intravenous injection there ap- 

 ])eared a typical reaction which somewhat extended twenty-four 

 hours later. Healing was very slow. The phenomenon \\as elicited 

 in about 50 per cent of animals tested with the afore-described 

 material. After maintenance, the strain lost its ability to produce 

 the active principles. In addition, one more strain of B. abortus 

 yielded potent preparations whilst tAvo other strains gave entirely 

 negative results. 



ACTIVE PRINCIPLES OF VARIOUS MEMBERS OF HEMORRHAGIC 

 SEPTICEMIA GROUP 



In 1929, filtrates of B. suisepticiis and B. avicida cultured for 

 six days in tryptic digest broth and on agar for t^venty-four hours 



