44 LOCAL TISSUE REACTIVITY 



\erscly, B. influenzae and B. perhissis when injected intravenously, 

 elicited reactions in rabbit skin prej)ared twenty-four hours pre- 

 \iously with B. Ixphosus "agar washings" filtrates. 



ACTIVE PKINCn'LKS OF VHiRIO CHOLERAE 



In H).'^o, I elicited the tyj)ical reactions with filtrates cjf cultures 

 ol cholera \^ibri() on ])lain agar, pH 7.8; 0.25 c.c. of undiluted 

 material was used for the intracutanecjus injection and 1 c.c. of 

 the same luidiluted material for the intravenous injection. 



Ciratia and Linz {icy^^^c) worked with filtrates of six day old 

 j)lain brotii cidtines. Tiie material was employed for reproduction 

 of tiie jjhenomenon in guinea pigs. Three-tenths c.c. of the filtrate 

 was used fcjr the jireparatory injection and i c.c. for the intra- 

 cardial injection, twenty-fom' horns later. 



Saburo Uyeda (1934) studied the effect of filtrates derived from 

 J' i brio cJiolerae cultures groAvn on plain agar slants for 24 hours. 

 The growth was suspended in 0.5 c.c. saline solution (5 c.c. to an 

 agar slant) . The emulsic^ns were then centrifuged and the super- 

 natant fluid filtered through a Berkefeld "V" candle. Six out of 

 8 strains yielded potent preparations. Apparently, large amounts 

 were necessary for elicitation of the reactions. Similar prepara- 

 tions made from cidtures of Vibrio El Tor, Vibrio melchiiikovi, 

 Vibrio albensis and Vibrio tyrogenum were also able to produce 

 the phenomenon. Cidtme filtrates of Vibrio a(j)ialilis proved in- 

 active. 



Vassiliadis (1935) employed filtrates of six day old broth cid- 

 tures of Vibrio cholerae and the El Tor vibrio for the elicitation 

 of the phenomenon both in rabbits and in guinea pigs. He w^as 

 able to elicit reactions in guinea pigs with either of the prepara- 

 tions. Whilst cholera vibrio cidtme filtrates produced the phe- 

 nomenon in rabbits, those of El Tor vibrios failed to do so. In 

 a recent investigation Linton, Singh, and Seal (1935) were also 

 successful in producing the phenomenon ^vith active principles 

 of Vibrio cholerae. Twenty-four hour old broth cultures served 

 lor inocidation of Roux flasks containing agar in amounts of 10 

 to 12 c.c. to a flask. Twenty hour old gro^vth was scraped into the 

 condensation water, filtered through Kieselguhr and Chamber- 

 lain L3 candles. (Seitz E. K. discs removed the active principles.) 

 The phenomenon ^vas elicited by an intradermal injecticMi of 

 0.25 c.c. and by an intravenous injection of 1 to 1.5 c.c. The 

 lesions were extremely severe, healing requiring about six weeks. 



