REACTIVITY TO VARIOUS MICROORGANISMS 49 



comparati\e data are a\ailal)le as to the exact potency of these 

 preparations. 



More extensixe inxestigations \vere done with ciihmes and cid- 

 ture lihrates of Streptococcus viridcnis. The experiments l)elonged 

 to the three groups above entnnerated. The strains employed were 

 either old stock or recently isolated from the blood stream of cases 

 of sni)aciite bacterial endocarditis. In early experiments a recently 

 ir.olated strain ol Streptococcus viridans (Joel) xvas cnlttn'ed aero- 

 bically, partially anaerobically and anaerobically (inider a vase- 

 line seal) in 2 per cent glucose broth of pH 7.2 and incubated 

 for one day. The skin sites were j^repared xvith potent heterol- 

 ogons filtrates. 



Twenty-fotn- liotns later, the abo\e described li\e cidtmes of 

 Streptococcus virida)is were injected intravenously in a d(jse of 

 '>, c.c. ])er kilo (^f body xveight. Strongly jDositive reactions were 

 obtained in abotit 40 per cent of rabbits tested xs ith these prepara- 

 tions. Niniierons attempts were also made to obtain potent Strep- 

 tococcus viridans principles in filtrates. \^arious methods of cid- 

 tivation were employed. The ctdtures were made in aerobic and 

 anaerobic double strength meat infusion broth and such stigars 

 as maltose and glucose were added daily in small amotuits (0.1 

 per cent) . Rabbits were prepared either xvith homologous or 

 heterologous bacterial filtrates. All the residts proved negative. 

 In sid)sequent experiments a strain of Streptococcus viridans 

 was grown in brain infusion broth containing Difco proteose. 

 The ctdtine xvas incubated for foiu' days. The filtrate thtis obtained 

 was tested by intravaneous injection in a dose of 3 c.c. per kilo of 

 JDody Aveight, into rabbits previously prepared by an intradermal 

 injection of heterologous bacterial filtrates. Strong reactions oc- 

 curred in only a small percentage of rabbits. From the above de- 

 scribed and similar experiments, it seems that the actixe principles 

 of Streptococcus viridans occtn^ in a loxv concentration and that 

 processes of filtration inrpair their activity. 



Reactions with Streptococcus Schottm'uller : 



One strain of a strictly anaeroliic streptococctis, recently isolated 

 from a case of ptierperal sepsis, Avas tested. The strain xvas an ac- 

 tive gas producer. The ctdttnes yielded a foiU odor. A Florence 

 flask containing cooked lixer mediimi was inocidated xvith a 

 twenty-fom" hoin^ old culture of the organism, sealed "with vase- 

 line and inctibated for fom- days. The material xvas then centri- 



