REACTIVITY TO VARIOUS MICROORGANISMS 57 



and used for a period of approximately three to fotir weeks fol- 

 lowing the preparation. No preservative ^vas added. 



Rabbits ^vere prepared by potent meningococcus and B. typho- 

 sus "agar washings" filtrates. The above filtrates of gram-negative 

 anaerobic bacilli injected intravenously into these rabbits elicited 

 severe reactions in approximately 80 per cent of animals tested. 

 The preparations were apparently devoid of skin-preparatory po- 

 tency because the skin preparation of rabbits with potent heterol- 

 ogous filtrates was essential. Filtrates of Bacterium melaninogeni- 

 cum gro^vn in symbiosis ^vith Streptococcus gamma and those of 

 leptothrix possessed both skin-preparatory and reacting factors, 

 but of comparatively lo^v concentration. 



ACTIVE PRINCIPLES OF YEAST 



Bock (1932) reports that he was able to reproduce the phenom- 

 enon of local skin reactivity with filtrates of four day old cultmes 

 of yeasts in glucose broth. Reactions were obtained in rabbits re- 

 ceiving preparatory injections of these filtrates and subsequent 

 intravenous injections of B. coli culture filtrates; and vice versa, 

 when preparatory injections of B. coli culture filtrates were fol- 

 lowed by intravenous injections of the yeast filtrates. The reactions 

 were characteristic of the phenomenon although some^si^at pro- 

 longed in their appearance. Similar experiments with actinomyces 

 and a trichophyton [Achorion Sclwenleini) failed. The observa- 

 tions on the potency of yeast extracts Avere corroborated by I\'ano- 

 vics (1934). 



Inasmuch as yeasts are kno^vn to li\'e in symbiosis with bac- 

 teria, the possibility is not excluded that the active principles of 

 the phenomenon could have been due to the presence of the 

 symbiotic bacteria. 



ACTIVE PRINCIPLES OF ASCARIS LUMBRICOIDIS 



J. \V. Mu (1935) Studied the phenomenon to Ascaris lumhri' 

 coidis. Before extraction, the ascaris worms were ^vashed in run 

 ning water, immersed in 10 per cent formalin solution for 10 min- 

 utes and then again washed in running ^\ater. The extract Avas 

 prepared by placing 4 gms. of the whole ascaris ^vorm in mixture 

 of one part of Coca's solution, and 3 parts of normal saline for 

 two weeks. The extract ^vas shaken from time to time. After the 

 extraction, the mixture was filtered through one layer of filter 

 paper and 0.25 c.c. of concentrated phenol was added to each 100 



