150 LOCAI. 1 ISSUE REAC'IIVITY 



with liliratc's ol B. Icjji.sejjliciis ol low plR'n()iiien()n-j)i()(liu in;^ jm)- 

 tc-iuA, j)i()l)al)ly due to tlie spontaneous inlections ol rabbits Avitli 

 this organism (Hanger. i<)L'<S//) . In these and other ex})erinients 

 Apitz was luiable to lind any eorrehition between the primary al- 

 lergic resjjonse of the rabbits to this product and production ot 

 the j)henonien()n. 



Whilst prej)arations may be obtained, which in proper dilu- 

 tions fail to j)roduce any visible primary reaction; on tiie con- 

 trary, many diverse bacterial products, live cidtures of certain 

 microorganisms, and viruses producing local lesions of varying 

 intensity, fail completely to elicit the state of reactivity of the 

 phenomenon under consideration. This point could be ^vell illus- 

 trated by numerous experiments with heat-killed, autolyzed and 

 live cultures of Styej)tococcus vir'uUuis, .staljliylococcus, emulsions 

 of poliomyelitis monkey cord, Shope tumor suspensions, Rous 

 tinnor suspensions, etc. 



As will be described in Chapter vn, it is possible to elicit tbe 

 phenomenon by combined injections of live cultures of vaccinia 

 virus, B. tuberculosis, Streptococcus hemolyticus , B. anthracis, etc., 

 ^vith heterologous bacterial filtrates of ascertained phenomenon- 

 producing potency. It is of special interest that the reactions are 

 elicited only at certain stages of the evolution of the local infec- 

 tion (erythematous stage of vaccinia virus infection. Gratia and 

 Linz, 193U/). Advanced lesions, as a rule, do not react to the 

 provocative intravenoirs injection of active principles of the phe- 

 noinenon. 



As discussed before, the active principles can be destroyed by 

 prolonged heating or they may be completely inactivated by addi- 

 tion of immune serimi. 



It will be seen from Cierber's histological studies that bacterial 

 filtrates completely inactivated by addition of immune antisera 

 produce as much, or possibly a little more, primary inflanmiatory 

 reactions than properly diluted active bacterial filtrates or than 

 mixtures of active bacterial filtrates ^vith amoimts of normal sera 

 insufficient to neutralize the phenomenon-producing j^rinciples. 



Moreo\er, the lack of correlation between the inflammatory re- 

 action and production of the phenomenon is finther supported l^y 

 the evidence that rabl)its resistant to the phenomenon may give 

 primary reactions just as se\'ere, or severer, than those suspect ii)le 

 to the phen(jmenon. The suscejDtibility of guinea pigs to the 

 phenomenon is only irregidar. Rats are completely resistant to it. 



