ROLE OF INFLAMMATION I57 



Urethane ethyl : 



Urethane ethyl was used in dilutions 1:100, 1:200, and 1:500. 



The substance was also used in mixture with B. typhosus "agar washings" 

 filtrate without effect. 



Calcium chloride: 



Dilutions 1:100, 1:200, and 1:500 of calcium chloride were tested. 



The substance in above dilutions was also re-injected into sites prepared 

 with B. typhosus "agar washings" filtrates at various intervals of time with- 

 out effect. 



Antispleen sera: 



Guinea pigs and a sheep were immunized with rabbit spleen, according to 

 the method described by Bedson (1921, 1922). The guinea pig anti-serum 

 showed precipitation Avith rabbit serum in dilution 1:128, and the sheep anti- 

 serum precipitated with rabbit serum in dilution 1:64. The intradermal injec- 

 tion of the serum produced reddening, swelling, and in some instances, small 

 necrotic and hemorrhagic areas. The injection of 50 and 100 units of menin- 

 gococcus, Group III, 44 B., "agar washings" filtrate at various intervals of 

 time following the intradermal injection, failed to enhance the primary 

 reactions. 



Substances studied by others : 



The inability of non-bacterial substances to elicit the state of 

 reactivity to bacterial filtrates was amply corroborated by a large 

 group of various investigators who studied a number of the 

 substances described ab<)\e and also substances not previously 

 tested by me, namely: 



Plain broth (Hanger, 1928/^; P. Bordet, 1936^; H. Gross, 193 1«, 

 b, c\ and Stolyhwo, 1935, 1936) ; turpentine (Hanger, H. Gross, P. 

 Bordet, and DeCour, 1934) ; India ink (Gratia and Linz, 1932c; 

 and P. Bordet) ; peptone (P. l^ordet) ; horse serum (Gross and 

 Bordet) ; milk (Gratia and Linz; P. Bordet) ; agar (Sickles, 1931, 

 1934) ; gum tragacanth (P. Bordet) ; 20 per cent sodium chloride 

 solution (Aitoff, Dion, and Dobkevitch, 1936^/, b) , etc. According 

 to P. Bordet, peptone and milk seemed to ser\'e as skin-preparatory 

 factors in the following modification: 



(iuinea pig or rabbit serum was injected intracutaneously into 

 rabbits. T^venty-four hours later, there Avas local sAvelling or thick- 

 ening of the skin into Avhich 0.5 to 1 c.c. of peptone ^vater (2 per 

 cent Chapoteaut peptone) Avas injected intraciuaneously. Five 

 hoins after the peptone injection, 1 c.c. of B. coli filtrate injected 

 intravenously elicited a local hemorrhagic reaction. The control 

 sites injected with peptone alone or serum alone Avere negative. 

 The same results could be ofjtained Avitli milk \vhen used instead 



