ROLE OF INFLAMMATION 1 59 



ments she also failed to induce the state of reactivity with frac- 

 tions of agar obtained l)y partial acid hydrolysis. 



I failed to induce the state of reactivity by intradermal injec- 

 tion of 0.5 per cent of granulated and shredded agar in 0.(85 P^^^ 

 cent salt solution. 



Stolyhwo (1935, lO'^G) produced traimia in the skin of rabbits 

 by shaving" with a didl razor, application of ligattne, clamp, etc. 

 Twenty-foiu' horns later, the rabbits injected intravenously with 

 potent bacterial filtrates showed no reactions at the trainnatized 

 sites. 



Gratia and Linz (1932c) failed to elicit the state of reactivity 

 with distilled w^ater and a 20 per cent sodium chloride solution. 



Fretnid (ig'^^a) injected guinea pigs intracutaneously with 0.2 

 c.c. of an active B. typhosus culture filtrate and follo^ved this by 

 intracardiac injection of the same filtrate in a dose of 1 c.c. None 

 reacted with hemorrhage. He agrees, therefore, with H. Gross that 

 the phenomenon of local skin reactivity cannot be elicited in 

 guinea pigs with any degree of regtdarity, and states that condi- 

 tions necessary for the phenomenon are different in the guinea 

 pig and the rabbit. In spite of this statement, he proceeded w^ith 

 a group of experiments on the phenomenon in the guinea pig 

 and drew^ certain conclusions concerning the natiue of the phe- 

 nomenon in rabbits on the basis of his experiments in gtiinea 

 pigs. He reported that Avhen silver nitrate \vas injected into the 

 skin of guinea pigs, redness, edema, and necrosis follo^ved, and 

 in a few guinea pigs, small areas of hemorrhage cotild also be 

 noted. Abotit one-half of the gtiinea pigs ^vhich recei\ed an intra- 

 vasctdar injection of typhoid filtrate, reacted with hemcjrrhages 

 in the site of the injecticjn cjf siher nitrate. As described cjn page 

 155, silver nitrate ^vhich produces a primary severe hemorrhagic 

 necrosis has absolutely no skin-preparatory potency in rabbits 

 (Gerl)er, 1936^/) . 



Debonera, Tzortzakis and Falchetti (1932) described the skin- 

 preparatory effect of sterile \aseline in guinea pigs. According to 

 these authors, the intraxcnous injection of 1 c.c. of B. coli cidtine 

 filtrate elicits an immediate piuptnic reaction at the vaseline in- 

 jected sites. These experiments were repeated by me in rabbits 

 with entirely negati\e residts. Here again, inasmuch as the phe- 

 nomenon of local skin reactivity is doid^tftd and extremely irreg- 

 idar in guinea pigs, experiments of this nature cannot be consid- 

 ered in studies on the mechanism of the phenomenon in rabbits. 



