ROLE OF INFLAMMATION l6l 



tioii instilled into the eye produced a partial anesthesia of the con- 

 jimctixal reflex ^vithin one minute, and a complete anesthesia 

 \vithin three minutes following the application. The anesthesia 

 disapj)eared completely within fifteen minutes. Upon intradermal 

 injection, 5 per cent solution of alyj^in produced a local necrosis 

 without hemorrhage; 2.5 per cent solution of the preparation pro- 

 duced no necrosis but a slight inflammatory edema. Alypin ^vas 

 added to the bacterial filtrate used for the preparatory injection. 

 In other experiments, it was reinjected into the site prepared 

 with the bacterial filtrate one-half, one liour and twenty-four horns 

 follo^ving the preparation. The provocative injection of B. typlio- 

 sus "agar \vashings" filtrate was made t\venty-four hoins after 

 the preparatory intradermal injection of the bacterial filtrate. In 

 all the experiments alypin failed consistently to produce any effect 

 upon the phenomenon. 



Cocaine : 



Cocaine was used in 5 per cent and 2.5 per cent soliuions, sim- 

 ilarly to alypin. There was observed no effect of cocaine upon the 

 phenomenon luider discussion. 



Acetylcholine : 



The injection of acetylcholine in a dose of 1 mg. per kilo of 

 body Aveight, preceding the injection of provocative factors at va- 

 rious intervals of time, failed to produce any effect. 



Pyridine : 



Studies on the effect of pyridine ^vere of interest because of Bed- 

 son's observations on the ability of pyridine to reduce substantially 

 the number of blood platelets in rabbits; 0.06 gms. per kilo of 

 body weight of pyridine produced no lethal effect. Rabbits were 

 prepared with meningococcus "agar washings" filtrates, treated by 

 intravenous injections of pyridine t^vo, twenty-four, and forty- 

 eight hours follo^ving the skin preparation. Twenty-five reacting 

 imits of meningococcus "agar Avashings" filtrates ^vere injected in- 

 tra\'enously t^venty-foin- horns after the skin preparation. All rab- 

 bits showed typical strong reactions. 



Anti-platelet serum: 



(iratia and Linz (1932c) employed Roskam's anti-platelet 

 serinn in the follo^vino manner: 



