l()2 LOCAL TISSME REACTIVITY 



Rabbits wvrv prejjared l)y intiadeniial injection of B. coli cul- 

 ture (iltiale and fi\'e Iiouis later injected with the serum in a 

 dose of 1 c .c . B. coli cuhure filtrate injected twenty-ioiu" hours 

 alter (he skin pi ej)arati<)n. seemed to elicit unusually stroni^ re- 

 actions in the 2 rabbits tested. Inasnuu ii as the sjieed with which 

 the reaction ajjpears is sid)ject to indixidual variation, no con- 

 ( lusion can be drawn from this exj)eriment. 



As mentioned before, in my e\j)eriments. anti-spleen serum pre- 

 pared according to the methods of Bedsou, failed to elicit the state 

 of reactivity of the phenomenon. 



Prolan : 



Three intravenous injections of large amounts of ]:)rolan (7 c.c. 

 per kilo of body weight) given at tAventy-foin-hour intervals j^ro- 

 duced no effect upon the phenomenon. 



Rabbits treated with prolan \S'ere also injected with subminimal 

 doses of reacting factors. No enchanced susceptibility was detected 

 in these e\]Deriments. 



General anesthesia: 



Several grotips of rabbits were prepared ^vith meningococctis 

 "agar ^\•ashings" fdtrates. Twenty-four hotns later they were anes- 

 thetized. LInder anesthesia they ^vere injected intravenously with 

 25 imits of meningococctis "agar washings" filtrates. Anesthesia 

 was continued for one-half and one hour following the intravenous 

 injection. There ^vas observed no effect upon the reactions. 



India ink: 



Gratia and Linz (i932r) injected intraperitoneally large dosei 

 of India ink until the skin of the animals became intensely black. 

 The prcxedure did not enhance, nor reduce the stisceptibility to 

 the phenomenon. 



STUDIES ON THE BEHAVIOR OF CAPILLARIES IN SITES PREPARED WITH 



ACTIVE PRINCIPLES 



Histological investigations on the sites prepared with bacterial 

 filtrates prior to the injection of reacting factors do not demon- 

 strate any paralysis or unusual dilatation of capillaries of the area 

 prepared. For these reasons, it appeared of interest to Kielanowski 



