ROLE OF INFLAMMATION 163 



(1935) and Kielanowski and Selzer (1934//) to determine the re- 

 sponse of sites thus prepared to various physical and pharmaco- 

 logical agents. Their findings were as follo^vs: 



The increase of temperature by application of cotton tampons 

 dipped in hot water (50° to 75° c.) pro\oked hyperemia in the 

 prepared site similar to the reaction of normal skin. The elexation 

 of temperatme and the ensuing congestion did not cause the ap- 

 pearance of petechiae. The prepared skin and its capillaries re- 

 acted in a normal manner. Tiie elevation of temperature of the 

 prepared area in rabbits injected intravenously with a potent 

 cultiue filtrate accelerated slightly the appearance and course of 

 the hemorrhagic reaction. The congestion of tiie prepared site 

 produced by massage had the same effect. The injection of 0.5 

 c.c. of adrenalin solution (1:1000) into the prepared site caused 

 the appearance of an ischemic zone due to the capillary constric- 

 tion. The hemorrhagic reaction around this zone developed nor- 

 mally but there were no petechiae in the ischemic area. (Similar 

 observations were made by Klein, 1931-32.) If the adrenalin in- 

 jection Avas gi\'en when the petechiae started to appear, there 

 Avas a resultant anemia but no petechiae dexeloped in the ischemic 

 area. 



Injection of histamine in dilution of 1:1000 had the same effect 

 as adrenalin but the anemia ^vas more marked and lasted longer. 

 Injection of physiological saline had no effect on the appearance 

 and course of the phenomenon of local skin reactivity. The cooling 

 of the sensitized skin by ether had no effect on the reaction. The 

 authors concltided from their experiments that capillaries react 

 normally in the prepared skin sites. They believe, therefore, that 

 some specific biophysical ciiange takes place in the capillaries 

 through the effect of the j^rej^aratory injection of bacterial fdtrate, 

 which thus far, cannot be demonstrated by the ordinary studies on 

 capillary permeability and plnsiological bcha\'ior of the capillaries. 

 They also state that the hypothesis of formation of "jiurj^urogenic 

 factors" offered by Ciratia and Linz (i932r), lacks experimental 

 and morphological proof. 



In my own experiments, sites prepared with bacterial filtrates 

 uere tested by application of suction prior to the injection of 

 pro\'ocati\e factors and following it. This procedure did not dis- 

 close any enhanced fragihty. The same suction aj:)plied to the skin 

 after the injection of the provocative factors resulted in small 



