iGf) LOCAL TLSSUE REACTIVITY 



willi various niicr()()r*>anisiii.s and that (|iialitati\cly the lesions 

 were all the same irrespec ti\e ol the niiciooi^anisni enij^loyed. 

 According to this author, histoloj^ically, the primary reaetion to 

 the intradermal injection ol B. tyjjiiosus liltrates is irregular and 

 considerably less pronoiniced than lollowing the injection of B. 

 I('j)i.s('j)licu.s. The intensified tyj^e of primary reaction produced 

 with B. U'j)i.s('j)licus cultine idtrates is probably due to the addi- 

 tional complicating effect of spontaneous hypersensitiveness of 

 the rabbits to widely spread natural infection with this group of 

 organisms (Hanger) . Apitz draws attention to the possibility of 

 differentiating the hemorrhagic lesions typical of the phenom- 

 enon of local skin reactivity to bacterial filtrates {viz. B. ty- 

 pliosus, etc.) from the superiinposed liemorrhage in sites reacting 

 with primary allergic reaction (B. lepisepticus) . In his sttidies 

 on the primary reaction to B. lypJiosus, B. coli, and other active 

 principles of the phenomenon, he observed that the primary 

 reaction gixes rise to an acute leucocytic and edematous infiam- 

 mation which, in contrast to the primary allergic inffanmiation of 

 B. lepisepticus, begins to disappear in twenty-four hoins following 

 the injection. The appeaiance of this inflammation is entirely 

 non-specific and histologically, totally unrelated to the reaction 

 following the injection of the provocative factors. The reaction 

 of the jjhenomenon is described by him as severe damage of all 

 the blood vessels of the reactive area, the main symptom being 

 profirse hemorrhage. The vascular damage is made obviotis by 

 the migration of leucocytes through the vessel ^vall, conspicuous 

 formation of parietal j)latelet throml)i, severe dilatation of lilood 

 vessels accompanied loy k.aryorrhexis and fatty degeneration of the 

 endothelial cells. This severe reaction is followed by necrosis lead- 

 ing to demarcation and separation of the destroyed tissue. 



Kielano^vski (1935) and ICielanowski and Selzer (1934/;) 

 studied morj)hological changes in rabbits which were prepared by 

 two simultaneoirs intradermal injections of 0.5 c.c. of B. coli cul- 

 tine filtrate. This was followed by an intravenous injection of the 

 same filtrate twenty-two hours later. At intervals of twenty-two 

 hotns after the first injection (just before the intravenous injec- 

 tion) ; t^vo hoius and thirty-five minutes, four hc^ms and forty-five 

 minutes, three hours and fifteen minutes, four hours, four hours 

 and t^venty-five minutes, six hours and forty-five minutes, twenty- 

 loin hours, and sixty-five and one half hours after the second injec- 

 tion, sections were taken fcjr histological examination. Seven of 



