ROLE OF INFLAMMATION 177 



Twenty-foui hours laier. one oi the sites was reiiijectecl with the 

 B. tyfjliosiis filtrate; another site was ireiiijected with one oi the 

 folloAving substances: 0.5 per cent oimi tragacanth, 10 per cent 

 gelatine, 0.2 j)er cent potassimn alinn, undiluted liver extract 

 and histamine hydrochloride diluted 1:2000; and a third. site was 

 injected Avith a niixttne of the B. typhosus filtrate with one of 

 the above sul)stances. T\\enty-fotu' hotns later in some areas thus 

 treated there Avas noticed inflanmiation of varying intensity and 

 also edema. No typical reactions were obtained. At thisti'me, the 

 rabl)its were injected intra\enously Avith_ 100 reacting tmits of 

 B. typhosus "agar Avashings" fUtrate. Many sites de\eloped tyj)ical 

 reactions fotu" horns after the intravenous injection of the react- 

 ing factors. Exceptions ^vere noticed in areas showing extensive 

 inflammation and edema. As may be seen, the repeated intra- 

 dermal injections of bacterial fdtrates alone in mixttires with 

 \arious materials, ^vhich may be expected to delay the absorp- 

 tion of the toxic fdtrates, failed to produce reactions typical 

 of the phenomenon wdien no provocative intravenous injection 

 Avas made. Futhermore, in these experiments, as in many others, 

 it Avas suggestive that extensive primary inflammation is inhibitory 

 to the elicitation of the phenomenon. 



In attempts to produce the phenomenon by repeated intrader- 

 mal injections the interval of time between the injections was 

 also varied (t^vo, fotn, six, t^venty-four, and forty-eight hotns) . 

 The results were consistently negative. In other experiments 

 sites prepared by intradermal injection of B. typhosus "agar wash- 

 ings" filtrates tAventy-four horns later were reinjected with sus- 

 pensions of monkey poliomyelitis virus, Shope fibroma and Shope 

 jDapilloma sus}5ensions, Streptococcus hemolyticus broth cidtine 

 filtrates, etc. In no instance Avere there observed any reactions 

 similar to those of the phenomenon of local skin reactivity. 



Frisch, H. Gross, and Gratia and Linz failed to elicit any reac- 

 tions Avhen pro\'ocative injections Avere given sid)cutaneously, in- 

 tracutaneously, and intramusctdarly. Frisch also fed large amoinits 

 of B. typJiosus filtrates by m(nith Avithout success. Frisch, H. Gross, 

 (ic)l\ia, b, c) , Gratia and Linz (1932c) , and P. Bordet (iQ.S^i/^) , 

 however, succeeded in reproducing the j)henomenon Avhen the 

 jjro\'ocati\'e injection Avas gi\en intraperitoneall) in large doses. In 

 the experiments of Frisch, four hours after the intraperitoneal in- 

 jections had been made, blue discolorations ajjj^eared at the site of 

 the previous skin injections in 4 otit of 12 animals tested. The dis- 



