ROLE OF INFLAMMATION 1 83 



A\ itli 200 reacting units of meningococcus and removed one-half 

 Iionr after the injection, were capable of eliciting reactions in 

 prepared rabbits. Higher dilutions of the extracts were lacking in 

 skin-preparatory and reacting potencies. TesticiUar and kidney 

 extracts yielded entirely negative residts. Apparently, a small 

 amount of the active principles injected into the blood stream 

 may be reco\ered from some organs. Titrations of the reacting 

 potency of the organ extracts thus obtained suggested, ho^vever, 

 that the amoiuit present may be that of the blood in the organs 

 remoxed and certainly do not indicate a concentration of the 

 actixe principles in any of the organs tested. Plant failed to elicit 

 the phenomenon xvith saline extracts of rabbits' testicles infected 

 with Spirodieta jjdllidd and also xvith the juice of the infected 

 testicles obtained by means of a press. 



Stolyhxvo (1935, 1936) performed the following experiment: 

 Eight rabbits received intravenously 3 to 5 c.c. of three different 

 batches of filtrates of a six day old B. typlwsus l^roth culture. Three 

 rabbits died xvithin the following ten to twenty-fom^ horns. Inci- 

 sions xvere made into the bladder and the mine collected. LIrine 

 cultures were sterile. Spontaneously excreted urine of the surviv- 

 ing rabbits was also collected txventy-four hoins after the intra- 

 venous injection. The mine xvas sterilized by boiling, inasmuch 

 as the active principles of the phenomenon are heat-stabile. The 

 urine samples of the dead and sm'viving rabbits were then in- 

 jected into 2 to 4 rabbit skin sites in amoimts from 0.2 to 0.3 c.c. 

 Txventy-four hours later, xvhen the prepared skin sites shoxs-ed 

 erythema of varying degrees and slight swellings, 3 to 5 c.c. of a 

 six day old B. typJwsus cultme filtrate xvas injected intravenously 

 into the prepared rabbits. Txvo rabbits died. Six surviving rab- 

 bits showed severe hemorrhagic and necrotic lesions in the pre- 

 pared skin sites. Control experiments xvith 6 samples of mine of 

 normal ral)bits gave negative results. 



The work of Stolyhwo suggests that the active principles in- 

 jected intravenotisly may be excreted by the kidneys. Apparently, 

 hoxvever, this author had to inject enormous amomits of the mate-, 

 rial in order to obtain the elimination in the mine. In Schneier- 

 son's experiments conducted in my laboratories, smaller amomits 

 of active principles of meningococcus and B. typJiosus ("agar 

 washings" fdtrates in doses from 500 to 1000 reacting units per 

 kilogram of body weight) failed to appear in the mine immedi- 



