Chapter VII 



METHODS OF ELICITATION OF THE PHE- 

 NOMENON OF LOCAL TISSUE AND ORCxAN 



REACTIVITY 



PERIVASCULAR REACTIVITY 



SHORTLY after the phenomenon of local skin reactivity to 

 I)acterial filtrates Avas described by me, attempts were made 

 to reproduce it in organs other than the skin. In these ex- 

 j^eriments approximately the same technique \vas followed, 

 ^vhereby the preparatory injection of a filtrate was made into the 

 parenchyma of an organ, followed twenty-fotir hours later, and 

 in some instances, at different intervals of time, by an injection of 

 active principles into the general circidation. 



In 1929, I reported on the phenomenon of reactivity in the 

 lungs of rabbits. A preparatory injection of 0.5 c.c. of B. typliosus 

 "agar 'washings" filtrate ^vas given intratracheally. Twenty-foin' 

 hours later, 1 c.c. of the filtrate was injected into the rabbit ear 

 vein. Large hemorrhagic and necrotic areas appeared through the 

 entire lungs of the animals thus treated. In the gross, the appear- 

 ance of the lesions \vas typical of the phenomenon in the skin. 

 Intratracheal injections alone jjroduced no macroscopic reaction 

 in the rabbit's lungs. 



Gratia and Linz (1932c) considered it of interest to determine 

 Avhether the phenomenon could be elicited in neoplastic tissues. 

 Liposarcoma in the guinea pig, which ordinarily is avascular and 

 is associated ^vith little sjjontaneous hemorrhage, ^vas selected for 

 the work. Young guinea pigs ^vere inoctdated with this tumor. 

 When, after several weeks, the tumors grew to the size of an egg, 

 an intratumoral injection of 0.3 c.c. of the B. coli active prin- 

 ciples Avas made. T^venty-four hours later, the prepared guinea 

 pigs received a provocative injection of 1 c.c. of the same prin- 

 ciples into the jugular vein or intraperitoneally. Some of the 

 guinea pigs died in a few days following the injection. Post- 

 mortem examination demonstrated an intense iiemorrhagic re- 

 action extending throtigh the major portion of the ttimor, sharply 



186 



