i88 LOCAL TISSUE reac:tivity 



tare filtrate (Ecker and Welch (1930) into the gastric mticosa; 

 and in other instances, into the mnscular layer of stomach of 

 rabbits. T^vo-tenths of the same (iltrate was injected intracuta- 

 neoLisly into the lower abdominal region. There were i() rabbits 

 of which 12 received 2^/, c.c. of the same filtrate intravenously 

 twenty-foiu- hours later. Animals were killed at intervals of three 

 and one-half, five, twelve, t\venty-foin% twenty-five, seventy-two 

 hours, and seven days after an intravenous injection. The tissues 

 were fixed in Zenker's fluid and stained with hematoxylin and 

 eosin. There were typical hemorrhagic and necrotic lesions in 

 the stomach ^vall, which ^vere generally parallel to the skin reac- 

 tions. The peak of the reaction occurred in the stomach twenty- 

 four hours after the intravenous injection. The local injection 

 into the stomach jjroduced an exudative inflammation, Avhich 

 after the intra venons injection became more severe and sho^ved 

 hemorrhage. The hemorrhage ^vas obtained only \vith both in- 

 jections, but in the stomach it ^vas not as severe as in the skin. 

 No ulcers ^vere observed. 



Moritz and Morley (i9^^i-^^2) reproduced the phenomenon of 

 local reactivity in the knee joints of rabbits. They nsed B. coli 

 and B. typliosus culture filtrates (Ecker and Rimington) . The 

 preparatory factors were injected laterally and belo^v the patella 

 with the knee joint flexed. One-tenth c.c. to 0.4 c.c. of the mate- 

 rial was injected. In most animals a simidtaneous skin injection 

 was made. Twenty to thirty hoius later, the intravenons injection 

 of 1 c.c. per kilo of body ^veight, ^vas given to the same animals. 

 There ^vere seven controls which received only the preparatory 

 injection. The animals were killed from one to one hnndred and 

 ninety-two horns follo^ving the intravenous injection. Six of the 

 10 rabbits tested showed a positive reaction in the synovial mem- 

 branes of the knee joints. The reaction ^vas less severe and oc- 

 curred less frequently in the joints than in the skin. Rabbits 

 prepared simultaneously in the knee and skin and showing severe 

 ctitaneotis reactions did not necessarily have severe synovitis and 

 vice versa. This observation may be explained by the fact that 

 several sites \vere prepared simidtaneously, and that possibly here 

 again an instifficient amoimt of provocative factors was given 

 intravenously to elicit the reaction in all sites prepared. The 

 authors' criteria employed for recognition of the reaction 'were 

 based on the histological sttidies, i.e., vascular damage associated 



