1()0 LOCAL TLSSIIE REACH IVLIY 



artixc })riii( i])lcs c ii( iilal iiij^ in the blood stream u|)oii the reactive 

 joints. 



Loi and ("-ardia (i9-Vl) c\])ciinicnted witli dcnciAatcd kidneys 

 ol rabbits. The nerxes ol the hiluin were cnt, the (aj)siile 

 stri])ped and the \cssels cnt. Ihrec-tenths ex. ol a j)()tent l)a( terial 

 Idtrate was injected into the kidney. Twenty to tuenty-tour hours 

 later, o.^^ c.c. ol the same liltrate was injected intra\enously. Six 

 rabbits were used lor these ex|)erinients. T\vo died tollowin^ the 

 inlraxenoiis injection and 4 rabbits were killed twenty-lour hours 

 alter the second injection. Postmortem examination showed swell- 

 ing (due to decapsulation) , dilatation and necrosis smrounded 

 by a hemorrhagic zone. The reaction was more severe in animals 

 which died spontaneously. Histologically, there were observed 

 dilatation of the renal ttd)ides folloxving decapsidation, and also 

 necrotic areas, leticocytic and hemorrhagic foci in the siuToiuid- 

 ing parenchyma zones. In the limiina of the dilated vessels the 

 red blood cells were agglutinated (thrombi) and an ischemic 

 infarct was observed in one case. Controls were negative. The 

 authors asstmied that the denervated organs show a high suscepti- 

 bility to the phenomenon of local reactivity to bacterial filtrates. 



Inasmuch as during the txventy-fotir hoiu" interval betxveen the 

 operation and the intravenotis injection there should be suffi- 

 cient time for complete degeneration of the terminal nerve sup- 

 ply, Loi and Cardia think that the degeneration accompanied by 

 vasoparalysis favors the prodtiction of the phenomenon in resist- 

 ant animals. 



It does not seem that the number of animals employed in the 

 authors' experiments jtistify a comparative estimation of the sus- 

 ceptibility of normal and operated animals. It is ob\ious, how- 

 ever, that denervation does not interfere with the production of 

 the lesion. 



Koplik (1936) reported that the state of reactivity can be 

 elicited in the lymph nodes by means of an intralymphatic prep- 

 aration Avith the active principles of the phenomenon. In his ex- 

 periments amounts of 0.1 c.c. to 0.3 c.c. of B. typhosus "agar 

 xvashings" filtrate were injected into the circimiflex abdominal 

 lymphatic in the direction of the femoral vessels. After the filtrate 

 Avas injected into the lymphatic, the skin incision \vas closed \vith 

 silk. Txventy-foiu' hours after the initial injection the animals 

 were given an intravenous dose of 5 to 25 B. typhosus reacting 

 iniits, per kilo of body xveight. The animals were killed loin" to 



