METHODS OF ELICITATION OF PHENOMENON I9I 



six hours alter the intra\enotis injection and aiitopsied. Control 

 animals received the intralymphatic injection alone. The experi- 

 ments of this author clearly indicated that intravenous injection 

 ot a potent bacterial filtrate elicited strong hemorrhagic reactions 

 typical of the plienomenon in lymph nodes of rabbits prepared 

 by an injection of the filtrate into the afferent lymphatics. 



Single intralymphatic or single intravenous injections of the fil- 

 trates ^vere ineffective in evoking such changes. The state of local 

 reacti\'ity in the lymph nodes was limited to those nodes which 

 recei\'ed the direct effect of the filtrates. The reactions were simi- 

 lar to those obtained in lymph nodes adjacent to the prepared 

 skin sites (p. 17) . 



In Gronchi's (1934) experiments, 20 guinea pigs averaging 

 265 gms. in Aveight, received a preparatory injection of B. coli 

 ctdture filtrate into the left adrenal folloAved by an intraperi- 

 toneal injection of 0.1 c.c. of the same filtrate tAventy-foiir hoins 

 later. Histologically, in some guinea pigs there Avas observed a focal 

 reaction in the adrenals, which consisted of a zone of celhdar 

 necrosis. The red blood cells lost their staining affinities. The 

 non-injected right adrenal shoAved hyperemia, hemorrhage and 

 leucocytic infiltration in the cortex but there Avas no necrosis or 

 proliferation obserxed in the right cortex. In another group of 

 experiments by this author, 15 guinea pigs received one prepara- 

 tory injection of B. coli ctdttne filtrate into the left adrenal and 

 two intracardial injections of the same filtrate twenty-fotu' to 

 forty-eight horns later. Some of the guinea pigs shoAved, macro- 

 scopically, hemorrhages in the adrenals. Microscopically, the 

 cortical layer Avas intensely hyperemic. There Avere numerotis ex- 

 tensive hemorrhagic foci, leucocytic infiltration, mitotic figures 

 in the zona fasciolata, and slight edema and hyperemia of the 

 medtdla. 



Cassuto (i933«) gave the preparatory injections into the con- 

 jinictival tissue of rabbits. Macroscopically, and histologically, 

 lesions characteristic of the phenomenon of local skin reactivity 

 Avere observed. Accordino- to this author the octdar tissue is more 

 sensitive than the skin for the production of the phenomenon 

 luider discussion. 



Reitano and Loi (1934) produced the phenomenon Avith B. 

 lypJiosus filtrate in the pancreas of rabbits. The preparatory intra- 

 parenchymal injection of 0.3 c.c. Avas folloAved by the intravenous 

 injection tAventy-four hours Later. The animals Avere killed tAventy- 



