192 LOCAL TISSUE REACTIVITY 



lour lioms ahei the intravenous injection. I'Our ol the animals 

 showed heinoiiha^i( neciosis ol the lobes oi the pancreas. Con- 

 trols. \vhi(h were injected into the j^ancreas ^vith saline and re- 

 eeived an intravenous injection ol the B. lyjjhoMi.s liltrate twenty- 

 loin hours later, remained negative. The appearance of the 

 hemorrhages was similar to that ol necrotic pancreatitis in man. 



Several attemjjts to reproduce the phenomenon of reactivity in 

 the brain consistently failed, ((iratia and Linz, and others) . Plant 

 (19^52) obtained no lesions in the meninges following combined 

 intracisternal and intravenous injections of active principles of 

 Sj)i)<)cliet.a pallida (dialyzed culttne filtrates) . 



Bcjck (1932) made preparatory subdtnal and intracerebral in- 

 jections of active principles of the phenomenon and then gave 

 provocative intravenous injections after a suitable interval of 

 time. The subdmal preparation alc:)ne and in combination with 

 the provocative injection gave no lesions. The combination of the 

 intracerebral preparatory injection with the intravenous provoca- 

 tive injection of the hltrate elicited paralysis without fail. Some 

 inflammatory reaction follcjwed the intracerebral preparation 

 alone. The paralyzed animals showed no hemorrhage in the gross 

 or micrcjscopical ly . 



Thus, the ^vork described demonstrates the possibility of elicita- 

 tion of a state of reactivity by means of a preparatory injection 

 of an active bacterial filtrate in the skin and parenchyma of various 

 tissues and organs. While it is necessary that the provocative 

 injection be given by Avay of the blood stream, the state of reac- 

 tivity may be accomplished by means of a local preparation. 



As clearly seen from Chapter vi, the conspicuous lesions of 

 the phenomenon are thc^se of severe vascular damage. Apparently, 

 the reactivity is elicited by means of a contact of the preparatory 

 factors with the outside ^vall of the small blood vessels for a certain 

 period of time. For convenience, the method of elicitation of 

 reactivity in the skin, by means of intradermal injection; and in 

 internal organs, by means oi intraparenchymal injection, may be 

 termed as "local perivascular preparation." The question re- 

 mains, however, ^vhether a state of reactivity could be elicited 

 by Avay of the vascidar system. The experiments in this directic^n 

 could be divided into those in ^vhich a state of reactivity is in- 

 duced in a given organ by way of its local vascidar supply, i.e., 

 "local vascular preparation"; and those in which a state of reac- 

 tivity is elicited in an organ or organs through the introduction 



