ig4 LOCAL TISSUE REACTIVITY 



ol tinie. These preparatory injections Tailed. Iiowexer, to elicit 

 the state of reactivity of the phenomenon under discussion. The 

 same doses oixcn intradermally elicited the state in most rabbits 

 tested, llnlortiniately, larger doses ccjuld not be used because of 

 the lethal effect of these preparations. It was asstmied, then, that 

 the j^erivasciilar preparation was an essential prerecjiiisite for 

 elicitation of the state of reactivity. In experiments such as the 

 above, imder conditions of normal resistance, the capillaries may 

 not allc:>^v the diffusion of the preparatory factors into the tissues 

 Avhen given intraxenously. In vie\v of this assimiption, it ^vas 

 decided to accompany the preparatory intra\enous injections of 

 the active principles by varic^us agents known to modify capillary 

 permeability, as follo^vs: 



Preparatory hitravoious hijcctioiis of Active Prnicijjles Accom- 

 panied by CJiilling. The left ears of rabbits 'were chilled by 

 means of ice fjags applied to both sides for a period of ten minutes 

 prior and following the preparatory intravenous injection of 

 eighty reacting tmits of meningococcus Group III (44B) "agar 

 washings" filtrates. The clamps ^vere kept on for two minutes 

 following the intravenous injecticm. Distinct hyperemia followed 

 the treatment. The injection failed, ho^vever, to elicit the state of 

 reactivity. 



Preparatory Intravoious Injections of Active Principles in 

 Xylol-Treated Ears. The left ears of rabbits ^vere clamped off at 

 the base and rubbed with xylol until the veins became very 

 prominent. Immediately afterwards, rabbits received 50 reacting 

 units of meningococcus, Group III (44B) "agar washings" fil- 

 trates into the marginal veins of treated ears. The clamps w'ere 

 removed five minutes after the intravenous injections were com- 

 pleted. Twenty-four hours later the ears appeared s^vollen and 

 one was slightly hemorrhagic along the marginal vein. The pro- 

 vocative injection of active filtrate elicited reactions in 2 out of 

 8 rabbits thus prepared. In one of these, the reaction Avas doidot- 

 ful and in another it represented an enhancement of a primary 

 hemorrhagic lesion follo^ving the preparatory treatment. The re- 

 sults of this experiment, therefore, remain inconclusive. 



Preparatory Intravenous Injections of Active Principles Com- 

 bined with Etliyl Urettiane, Acetylcholine, Pilocarpine Hydro- 

 cliloride, Atropine, Calcium Gluconate and Guinea Pig Liver 

 Extract. In this series of experiments preparatory injections were 

 made intravenously in combination with the follo^ving sub- 



