METHODS OF ELI CITATION OF PHENOMENON 1 97 



scopically, there was pronounced thrombosis in the venules. 

 There were also observed petechial hemorrhages in various parts 

 of the ear and sometimes in portions far removed from the site 

 of the injected vein. In the gross, these petechiae closely resem- 

 bled pinpinic spots seen in the skin of hmnan cases of menin- 

 gococcemia. 



Ears exposed to 45° c. for fi\'e minutes ga\e definite reactions 

 following the provocative injection, provided at least 50 reacting 

 iniits were used for the preparatory intravenous injection. When 

 exposure to 50' c. ^vas combined \vith the preparatory injection 

 of active principles, distinct reactions were seen with as little as 

 5 iniits, provided the provocative injection was given into the 

 same vein. It is cmious that a preparatory dose of 25 reacting 

 units in ears exposed to 50° c. failed to elicit the state of reac- 

 tivity. This observation is difficult to explain and the experiment 

 shoidd be repeated. Provocatixe injections of acti\e principles 

 into the ears prepared by larger doses {i.e., 50 and 80 reacting 

 units in ears exposed to 50° and 55° c.) gave intense and diffuse 

 reactions. 



It may l)e concluded from the above tiiat j^reparatory injec- 

 tions of active principles are capal)le of eliciting the state of reac- 

 tivity in the skin of the ear provided they are combined with 

 temporary stasis (clamping) and thermal hyperemia. 



Preparatory Intravowiis Itijections of Active Principles Com- 

 bined ivitli Testicular Extract. The rabbit testicidar extract em- 

 ployed Avas prepared according to the method described by Duran- 

 Reynals (1933/;) . In one group of experiments the extract was 

 injected into the dermis in the vicinity of marginal veins of 

 clamped and non-clamped ears. Immediately afterwards, a prepara- 

 tory injection of meningococcus and B. typhosus acti\e principles 

 in various doses was made into the marginal vein of the treated ear. 

 The clamps were released fixe minutes after the intravenous in- 

 jection Avas completed. In another group of experiments menin- 

 gococcus and B. typhosus active principles Avere injected in mix- 

 ture Avith \arious amounts of testicidar extract. 



It Avas concluded from these experiments that mixtines of bac- 

 terial filtrate and testicidar extract are capable of inducing the 

 state of reacti\ity in the rabbit's ear by Avay of the vascular sys- 

 tem. The reactivity is elicited ])ro\ided the circulation is inter- 

 rupted for a fcAv minutes and the amount of filtrate is cjuite small. 

 It is suggestixe that the state may disapj^ear within four hours 



