METHODS OF ELICITATION OF PHENOMENON 1 gg 



hours is recjuiiecl lor tlie elicitation ol the state of reactivity may 

 be due to rehuive impernieal)ility ol the cells which does not 

 permit a rapid entrance ot the preparatory factors into them. The 

 role of the testicular extract and heat accompanying the intra- 

 vascular pre{)aration may, then, be two-fold; i.e., to allow the 

 passage of the injected substances into the siuroinidin^ tissues 

 through a rapid increase in the capillary permeability; and to 

 enhance the cell permeability. A shorter incubation period is, 

 therefore, sufficient for the preparation ^vith mixtines of the pre- 

 paratory factors ^vith testicular extract injected intravencjusly than 

 when the preparatory factors alone are injected intradermal ly. 

 As ])ointed out before, in all probability the state of reactivity 

 takes place in the elements of the vascular wall. It may be as- 

 sumed, therefore, that the natmal relative impermeability of the 

 cells of this wall does not attain the state of reactix ity unless the 

 preparatory factors remain in close contact ^vith them for a cer- 

 tain length of time. Under ordinary conditions of intravascidar 

 preparation, the contact of the preparatory factors ^vith the 

 \ascidar \\all may last cjnly as long as the circidation is stopped 

 and jjrobably may cease when the circulation is re-established. 

 If, ho^vever, a state of enhanced permeability is induced by means 

 of the testicular extract, a diffusion of the factors is allowed from 

 the \ascidar channel or possibly, the direct contact of even short 

 duration of the preparatory factors ^vith the permeable cells is 

 sufficient tC3 induce the state of reactivity (Sh^\■artzman i9^^5c) . 

 Alechinsky (ic);^^',, 19.^^6) made intravenous injections into ears 

 ligated at the fjase. The ligature ^vas removed 6 hours later. 

 Hemorrhagic reacticjns were obtained in the prepared ears when 

 a provocative injection of active principles was given sid3cuta- 

 neously, intramuscularly, intraperitoneally or intracranially. No 

 reactions appeared in ears ligated for the same period of time 

 but receiving no preparatory injections. It is obvious that in the 

 experiments of this aiuhor ligation for a period as long as 6 

 hours must have raised considerably the local \ascular permea- 

 bility and thus, similarly to my experiments, there Avas possible 

 the passage of the preparatory fact(jrs from the capillaries into the 

 perivascular tissue. \V^orthy of further study are the observations 

 of Alechinsky that reacting factors introduced sidocutaneously or 

 intramuscularly are capable of eliciting reactions in ears pre- 

 pared by way of the local vascular system. As was sho^vn before. 



