204 ix)c;al tissue reactivity 



^vere obtained Ijy Siet^niuiul, lol lowing the intraxenous injection 

 ol li\e c ultnies ol B. Ixjjho.siis. B. coli and stapliylococ c us. 



Effect of Repeated Intravenous Injections of Bacterial Fil- 

 trates: 



The follo^ving authors studied the lesions resulting from re- 

 peated intra\enous injections of acti\'e principles of the phe- 

 nomenon: 



Gratia and Linz (iq'^sc) injected 0.5 c.c. of B. coli active fil- 

 trate intra\enously into four rabbits. Two of these rabbits re- 

 ceived 1 c.c. of the filtrate intra\'enously twenty-four hours later, 

 xvhilst the remaining txvo rabbits received, during the folloxving 

 day, four injections of 1 c.c. of the filtrate diluted 1:1000, 1:100, 

 1:10 and of the undiluted material, respectively. On the day 

 following the completion of the experiment, one of the rabbits 

 of the first group and txvo rabbits of the second group, died. Post- 

 mortem examination showed blood in the peritoneal ca\'ity, 

 hemorrhagic lesions in the intestines, multiple petechial hemor- 

 rhages in the kidney, large circular hemorrhagic areas in the 

 lungs, as xvell as hemorrhages in the lymph nodes, thymus, and 

 bone marroxv. Apparently, no histological studies \vere made. 



Apitz (1934c/) 'was the first to make histological studies on the 

 lesions of internal organs in the phenomenon of reactivity by 

 way of the general circulation. He also deserves the credit for 

 first calling attention to pronounced vascular lesions in the kid- 

 neys. The intra\enous injections of B. coli "agar xvashings" fil- 

 trates xvere given by him at various intervals of time, i.e., txvo 

 injections at t^venty-four hour intervals or several injections at 

 intervals of five to six hours. The main kidney lesions described 

 by him were: (1) cortical necrosis, (2) focal glomerular tubular 

 nephrosis with and without infarction, (3) focal tubular nephro- 

 sis, and (4) glomerular nephrosis. 



In Gronchi and Carniellis' experiments (1934), guinea pigs 

 received two intracardial injections of 1 c.c. of filtrate of a seventy- 

 two hour old broth cultnre of B. coli. They w^ere killed four, six, 

 and twenty-four hours after the second injection. These authors 

 report histological studies only on the adrenals. The cortical sub- 

 stances shovved intense hyperemia, numerous focal hemorrhages, 

 and leucocytic infiltration, characterized by presence of mitotic 

 figures in the cells. The medullar substance \vas intensely hyper- 



