212 LOCAL ILSSIE REACTIVLIY 



tioiis ^vcrc obLiined in the small intestines, while no intestinal 

 lesions neie observed by Apitz and Cierber in the phenomenon 

 ol oeneral skin reactivity to bacterial factors alone, and that live 

 cholera vibrios were invariably isolated from the intestinal wall 

 by Sanarelli. Sanarelli also failed to produce the intestinal lesions 

 by combined injection of heat killed or autolyzed J'llnio cholerae 

 with B. coli or B. proteris culture filtrates. Moreover, the reverse 

 order of injections, i.e., B. coli ctiltine filtrate followed by injec- 

 tion of live I'ihrio cholerae cidture, oave little effect. He con- 

 eluded from his ex])eriments that there exists a definite selective 

 affinity of J'ihrio clioleiae for the intestines; that the reactions 

 described are anaphylactic in nature ("epithalaxie") reqiuring 

 sensitization with live organisms; and that the experimental \nc- 

 tine obtained is pathognomonic of hiunan cholera. 



COURSE OF INDUCED INFECTIONS IN ANIMALS PREVIOUSLY PREPARED 

 WITH ACTIVE PRINCIPLES 



Apitz (1934/;) injected li\e tAventy-foiU' hoiu" old Staph^lococ- 

 ciis aureus cultines into (1) normal rabbits. (2) horse serum- 

 sensitized rabbits, and (3) rabbits treated with active bacterial 

 principles of the phenomenon under consideration. 



Sensitization to horse serum was accomplished by a series of 

 sidjcutaneous, intraperitoneal, and intravenotis injections at five 

 to six days' intervals. Twenty-six to t^venty-eight days after the 

 last sensitizing injection, the rabbits received the last dose of 10 

 c.c. of horse serum intravenously and following it the injection 

 of Staphylococcus aureus. 



The active principles of the phenomenon employed for the 

 third group of rabbits were B. coli "agar washings" filtrates pre- 

 pared according to my method. 



Rabbits of Groups 1 and 2 developed multiple pyogenic foci. 

 The lesions varied in intensity and size. There ^vere seen small 

 abscesses in the heart muscle and kidney, as \vell as large abscesses 

 occupying the major part of the kidney. Whilst the pyemia \\as 

 of regtdar occurrence, no actUe endocarditis was seen as a ride. 

 The only heart lesions observed Avere thrombi of the chorda ten- 

 dinae of heart valves. In contrast, in the third group, the intra- 

 venous treatment of rabbits Avith the active principles of the 

 phenomenon twenty-four hours prior to the injection of staphylo- 

 coccus cultine, significantly modified the course of the staphylo- 

 coccus infection. The preliminary treatment Avith small doses of 



