2 14 LOCAL TLSSUE REACllVriY 



la\oral)ly iiilliiencc the course nl the iiileclion. The topic will 

 again receive eoiisideratioii in Chapter \ii. 



The aroie-inentioiied authors also inoculated the skin ol the 

 ear ol rabbits with vaccinia \irus by Friction. Fi\e clays later, 

 when the lesions consisted ol small isolated pustules, an intrave- 

 nous injection ol B. coli active princijiles was given. The lollop- 

 ing day the pustules weie simounded by a hemonhagic /one with 

 a necrotic center. It apjieared that the \accinia virus lesions were 

 aborted. Similar results ^vere obtained with rabbits inoculated 

 with the vaccinia virus into the skin of the thigh. The provoca- 

 tive injection ol the B. coli active j^rinciples was given three days 

 alter the inoculation with the virus, i.e., at the erythematous stage 

 ol the lesion. Shortly alter the intravenous injection, the erythema 

 gradually became darker. The lollowing day the entire area be- 

 came necrotic and assinned an appearance of tar. In this experi- 

 ment, the vaccinia virus lesions also failed to develop any fmther. 

 In another series of experiments, injections of the active prin- 

 ciples were given at five, eight, and nine days after the inocida- 

 tion of the vaccinia virus. The jjhenomenc^n seemed to have been 

 produced more irregularly at the fifth day of the infection while 

 it failed to appear altogether at the later stages. 



In further experiments of Gratia and Linz (1931^), vaccinia 

 virus was inoculated into the rabbit's testicles. Three days after 

 inoculation, when the testicles became edematous and donbled 

 in size, the rabbits received intravenonsly B. coli ctilture filtrate. 

 The animals died one to two days later. Postmortem examinaticjn 

 showed a severe reaction in the testicles consisting of bloody and 

 coagulated exudate. The hemorrhagic reaction followed the cord 

 of the inoctilated and non-inoculated testicles and invaded the 

 serotis membranes of the large intestines including the omentum. 

 The peritoneal cavity was filled wath blcjod. Inguinal and mesen- 

 teric lymph nodes were markedly hemorrhagic, also, the thynuis 

 and the lungs in one of the rabbits. Control rabbits receiving the 

 virus alone, showed the ordinary type of inflammatory reaction in 

 the testicles of the cord and peritoneum without any hemorrhagic 

 manifestations. No kidney lesions were observed. It is apparent 

 that the vaccinia virus possesses the property of inducing a state 

 of reactivity at a site of the primary inoculation, as well as in the 

 tissues of the secondary Icjcalization of the virus. Similarly to 

 Sanarelli's experiments with live bacteria, the state of reactivity 

 induced by live virus is conditioned by its selective affinity for 



