2l6 LOCAL ILSSUE REACTIVITY 



drop in tcnipeiature and death \vithin a few hours. Autopsy re- 

 \ealed heniorrliagic exudate in the peritoneal cavity and conges- 

 tion about the pseudo-tuberculous foci. 



In i()y,2 the observations were confirmed by Debonera, 

 Tzorkzakis, and Falchetti. These authors injected guinea pigs 

 subcutaneously with 0.25 c.c. of a light emulsion of Preitz-Nocard 

 bacillus, grown on serum agar. This amount caused either su])- 

 c utaneous abscesses which opened after ten days c:)r death by 

 toxemia in twelve days. Beginning with the thircf day cjf the in- 

 fection, the intravenous injection of B. coli culture filtrate pro- 

 duced a typical phenomenon at the site of the Preitz-Nocard l^acil- 

 lus infection. An incision at the end cjf twenty-four hours after 

 the injection of B. coli active principles showed that the process 

 extended to the skin and to the sulxutaneous tissue surrounding 

 the cavity of the abscess. On the third day there cjccurred elimina- 

 tion of pus and often healing. 



P. Bordet (ici^fV/) inoculated guinea pigs intraperitoneally 

 with r, mgms. of B. C. (i. From ten to twenty days later, these and 

 control guinea pigs were injected with B. coli in a dose of 1/10 to 

 1 '20 of a t^venty-foiu' hoin" old agar cidture. The B. C. G. treated 

 guinea j^igs died four to ten hours following the B. coli injection. 

 Some of the controls sinvived and others died at a later date. 

 The auto])sy of the experimental guinea pigs demonstrated a])un- 

 dant serous sanguinous exudate in the j^eritoneal ca\ity. The site 

 of the tul)erculoiis lesions in the mesentery ^vas highly congested 

 and showed niunerous ])etechiae. Similar cjbservations were made 

 by P. Bordet with killed cultmes of B. coli, ordinarily non-tc:)xic 

 to normal animals. In this series of experiments, guinea pigs 

 inoculated two to three ^veeks previously with 5 to 10 mgms. of 

 B. C. G. received an intraperitoneal injection of 2 c.c. of a sus- 

 pension of B. coli heated at 60° or 100° c. Three to four hours 

 after the injection the B. C. G. guinea pigs developed difficulties 

 in respiration, became prcjstrated and frecpiently died six to ten 

 hours following the injection, whilst the controls regidarly sur- 

 vived similar doses of B. coli heat-killed suspension. A dominant 

 symptom dining the course of this intoxication \vas a pronoiuiced 

 and rapidly progressing hypothermia accc:)mpanied by slo^ving of 

 the venous circulation. The temperature dropped from ,^58. 5° c. 

 before the injection to 34 to 35° c. three to four hours following 

 the injection and to 30° c. shortly before death. The control ani- 

 mals showed only a slight and transitory hypothermia. P. Bordet 



